microRNA-126对VEGF介导血管生成的调控作用  

Role of microRNA-126 on regulation of VEGF-mediated angiogenesis in vitro

作　　者：任梦宇 迟路湘[1] 欧书林[1] 

机构地区：[1]第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所,重庆400038

出　　处：《第三军医大学学报》2016年第20期2259-2263,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81270406)~~

摘　　要：目的研究microRNA-126(miR-126)对血管内皮细胞生长发育的影响,探寻miR-126可能参与的血管生成过程。方法常规培养脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)。构建miR-126过表达载体并转染HUVECs;实验分为:正常细胞组,空载慢病毒转染组,miR-126过表达慢病毒转染组(n=6);于转染第6天后用qRT-PCR检测内皮生长负性调节因子spred1、Pik3R2的mRNA表达水平,Western blot检测spred1、Pik3R2的蛋白表达水平。结果转染第6天后,对qRT-PCR及Western blot检测结果进行分析,过表达组中spread1、Pik3R2的2(-ΔΔCt)值较其余两组低;过表达组中spred1/GAPDH和Pik3R2/GAPDH值也均小于其余两组,差异具有统计学意义(P<0.05)。spred1、Pik3R2的mRNA表达水平明显下调,其蛋白表达水平也明显降低。结论 miR-126能促进VEGF的内皮细胞增殖及血管生成作用。Object ve To investigate the role of microRNA-126 （miR-126） on regulation of vascular endothelial growth factor （VEGF） mediated angiogenesis. Methods Human umbilical vein endothelial cells （HUVECs） were cultured and transfected with lentiviral vectors carrying miR-126. The＇ cells were divided into control group, empty vector group and miR-126 transfected group （LV3-miR-126）. Total RNAs were collected at day 6 after transfection. The mRNA expression of negative regulatory factors, sprouty related EVH1 domain containing 1 （spredl） and phosphoinositide-3-kinase regulatory subunit 2 （Pik3R2）, were measured by quantitative real-time polymerase chain reaction （qRT-PCR）. The protein expression of spredl and Pik3R2 was detected by Western blot. Results The qRT-PCR results showed that spredl and Pik3R2 were significantly down-regulated in LV3-miR-126 group compared with the control and empty vector groups （P 〈 0. 05）. Also, their protein expression levels were obviously decreased with statistical significance （P 〈 0. 05）. Conclusion miR-126 expression can promote proliferation of endothelial cells and VEGF-mediated angiogenesis.

关 键 词：MIR-126 内皮细胞 血管生成 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]