新疆地区维吾尔族人群高血压患者microRNA表达谱分析  

microRNA expression profile analysis of essential hypertension patients in Uyghur population

作　　者：叶远征[1] 周锐[2] 彭伊[1] 

机构地区：[1]新疆医科大学第一附属医院心脏中心心功能科,新疆乌鲁木齐830054 [2]新疆石河子市人民医院心血管内科,新疆石河子832000

出　　处：《基础医学与临床》2016年第10期1374-1380,共7页Basic and Clinical Medicine

基　　金：新疆维吾尔自治区自然科学基金(2014211C059)

摘　　要：目的筛选新疆地区维吾尔族人群原发性高血压(EH)患者与健康者血浆中microRNA差异表达谱,为进一步探究microRNA与新疆维吾尔族人群原发性高血压发病的关系提供依据。方法选取4例维吾尔族原发性高血压患者和4名维吾尔族健康人的全血,用Agilent Human miRNA芯片检测血浆microRNA表达谱。对差异表达的microRNA进行聚类分析、靶基因预测、靶基因注释等生物信息学分析。另外选取独立验证样本(15例维吾尔族原发性高血压患者及15名维吾尔族健康人),对差异表达的microRNA进行q PCR验证。结果实验组与对照组相比,筛选获得257个差异表达microRNA,其中161个上调表达,97下调表达。通过Target Scan、PITA、microRNAorg数据库对差异表达microRNA进行靶基因预测,对3个数据库预测到的靶基因取交集,获得6 580个microRNA调控的靶基因系。最终筛选得到表达上调最多和下调最多的两个microRNA,hsa-miR-1183和hsa-miR-30e-5p。结论原发性高血压患者有着独特的microRNA表达谱,筛选获得的差异表达microRNA,可能是原发性高血压早期诊断的生物标志物和潜在的治疗靶点,同时对于后续研究原发性高血压的发病机制具有提示作用。Objective To screen the microRNA expression file in Uighur population for exploring the relationship of microRNA and molecular mechanism of hypertension. Methods Whole blood samples were taken from 4 hypertension patients and 4 health people from Uighur population. microRNA expression profile was tested by using Agilent Human miRNA array. Clustering analysis and target gene annotation were performed on differentially expressed microRNAs. Another 30 subjects were sampled independently（ 15 from hypertension patients and 15 from healthy people） and the differentially expressed microRNAs were validated by quantitative PCR. Results microRNA profiles of the two groups,257 differentially expressed microRNAs were filtered,in which 161 were up-regulated and 97 were down-regulated. Target genes were predicted in three database,that is,Target Scan,PITA and microRNAorg. 6 580 target genes were obtained by computing the intersection of three databases. hsa-miR-1183 was the most up-regulated and hsa-miR-30 e-5 p was the most down-regulated microRNA in hypertension patients.Conclusions microRNA expression profile of experimental group was different from the control group. Differentially expressed microRNAs would be a potential biomarker for diagnosis or therapy. This study supplied a foundation for exploring the mechanism of essential hypertension.

关 键 词：原发性高血压 MICRORNAS MICRORNA表达谱 靶基因 

分 类 号：R544.1[医药卫生—心血管疾病]