黄体生成素及其受体在大鼠胰腺的免疫荧光定位  

Immunofluorescence localization of luteinizing hormone and its receptor in rat pancreas

作　　者：薛魁金[1] 马玉涛[1] 赵金钊 周连泉[1] 雷媛媛[1] 于辉[3] 

机构地区：[1]德州市人民医院消化内科,德州253014 [2]德州市中医院外一科,德州253022 [3]德州学院医药与护理学院,德州253023

出　　处：《解剖科学进展》2016年第5期510-512,共3页Progress of Anatomical Sciences

基　　金：山东省自然科学基金(ZR2011CL001)

摘　　要：目的观察黄体生成素(LH)及其受体(LHR)在SD大鼠胰腺的定位分布,以了解LH对胰腺的功能意义。方法选取SD大鼠12只,雌雄不拘,经腹腔注射戊巴比妥钠麻醉后,用4%多聚甲醛灌流固定2h,取出胰腺组织置于300g/L蔗糖液中,用于双标记免疫荧光定位研究,在激光共聚焦扫描显微镜下观察。结果胰腺部分外分泌腺细胞质、导管上皮细胞及部分胰岛细胞质LH反应阳性,小血管内皮细胞质呈LH弱阳性。部分胰岛细胞质呈LHR反应阳性,一些外分泌腺上皮细胞质呈LHR反应弱阳性,部分胰岛细胞及胰腺外分泌细胞质呈LH和LHR双阳性反应。结论LH可能通过旁分泌或自分泌的途径对胰腺的功能起调节作用。Objective To observe the localization of luteinizing hormone（LH） and its receptor（LHR） in the pancreas of SD rats. Methods Double label immunofluorescence histochemieal staining and confocal laser-scanning microscope were used to study the localization of LH and LHR in the pancreas of 12 SD rats. Results LH strong positive reaction was found in pancreatic exocrine portion, ductal epithelial cells and islet cells, but weak in small vascular endothelial cells. LHR strong positive reaction was found in some islet cells, but weak in exocrine gland epithelial cells. LH and LHR double positive reaction was seen in some islet cells and exocrine pancreas cells. Conclusion LH and LHR might be involved in the function of pancreas through paracrine or autocrine manner.

关 键 词：黄体生成素 黄体生成素受体 胰腺 免疫荧光组织化学 激光共聚焦扫描显微镜 SD大鼠 

分 类 号：R333.4[医药卫生—人体生理学]