PI3K/Akt/mTOR信号通路对大鼠肾小球系膜细胞增殖的调控作用探讨  

Regulatory Role of PI3K/Akt/mTOR Signaling Pathway in Rat Glomerular Mesangial Cell Proliferation

作　　者：张春江[1] 赵丹[1] 陈志刚[1] 杨晓萍[1] 

机构地区：[1]石河子大学医学院第一附属医院,石河子832008

出　　处：《内科急危重症杂志》2016年第4期308-311,共4页Journal of Critical Care In Internal Medicine

摘　　要：目的:探讨磷脂酰肌醇-3(PI3K)/蛋白激酶(Akt)/哺乳动物雷帕霉素靶蛋白(m TOR)信号通路对大鼠肾小球系膜细胞(MC)增殖的调控作用。方法:体外培养大鼠MC,随机分为正常对照组、表皮生长因子(EGF)(10ng/m L)组、PI3K抑制剂LY294002组(2μg/m L)、EGF联合LY294002组。采用四甲基偶氮唑蓝(MTT)法检测4组MC的增殖,流式细胞术检测MC周期,免疫荧光法检测系膜细胞m TOR的表达。结果:与正常对照组相比,EGF组能显著促进大鼠MC增殖,G0/G1期细胞减少,S期及G2/M期细胞增加,且m TOR表达增加;LY294002组大鼠MC增殖受抑制,G0/G1期细胞增加,S期及G2/M期细胞减少,且m TOR表达降低;与EGF组比较,EGF联合LY294002干预组MC增殖受抑制,G0/G1期细胞增多,S期及G2/M期细胞减少,且m TOR表达降低。结论:m TOR信号通路参与了大鼠肾小球MC增殖的调控,抑制该信号通路的活化可显著抑制MC的增殖和MC周期的进程。Objective： To investigate the regulatory role of PI3 K / Akt / m TOR signaling pathway in rat glomerular mesangial cell proliferation. Method： The cultured rat glomerular mesangial cells were randomly divided into four groups： normal control group,epidermal growth factor（ EGF）（ 10 ng / m L） group,LY294002（ 2 μg / m L,the inhibitor of PI3K） group and EGF plus LY294002 group. The cells were treated for 48 h. The cells proliferation was measured by MTT colorimetric method. The cell cycles were examined by flow cytometry. The expression of m TOR in each group was detected by immunofluorescence staining. Results： Compared with the normal control group,the proliferation of rat glomerular mesangial cells was significantly increased,the proportion of cells in G0 / G1 phase was decreased and that of cells in S and G2 / M phase was increased,and the expression of m TOR was increased in the EGF group; The proliferation of rat glomerular mesangial cells was inhibited,the number of cells in G0 / G1 phase was increased and that of the cells in S and G2 / M phase was decreased,and the expression of m TOR was decreased in the LY294002 group. Compared with the EGF group,the proliferation of rat glomerular mesangial cells was inhibited,the number of cells in G0 / G1 phase was increased and that in S and G2 / M phase was decreased,and the expression of m TOR was decreased in the EGF plus LY294002 group. Conclusion： m TOR signaling pathway takes part in the process of rat glomerular mesangial cell proliferation. Inhibiting the signaling pathway can significantly inhibit the proliferation and cell cycle of rat glomerular mesangial cells.

关 键 词：肾小球系膜细胞 EGF PI3K/Akt/m TOR信号通路 细胞增殖 

分 类 号：R692[医药卫生—泌尿科学]