CuO-ENPs在三疣梭子蟹不同组织中的积累效应研究  

Accumulation Effect Research of CuO-ENPs in Different Tissues of Portuns trituberculatus

作　　者：李铁军[1] 胡红美[1] 薛彬[1] 尤炬炬[1] 刘琴[1] 张玉荣[1] 喻亮[1] LI Tie-jun HU Hong-mei XUE Bin You Ju-ju LIU Qin ZHANG Yu-rong YU Liang(Marine Fishery Research Institute of Zhejiang Province, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds, Ministry of Agriculture, Key Laboratory of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Zhoushan Zhejiang 316021)

机构地区：[1]浙江省海洋水产研究所,农业部重点渔场渔业资源科学观测实验站,浙江省海洋渔业资源可持续利用技术研究重点实验室,浙江舟山316021

出　　处：《现代农业科技》2016年第19期259-260,265,共3页Modern Agricultural Science and Technology

基　　金：浙江省自然科学基金委青年科学基金(LQ14B070002)

摘　　要：为研究CuO-ENPs在三疣梭子蟹不同组织中的积累效应,选择三疣梭子蟹的鳃、血淋巴、肌肉、肝胰腺、心脏和胃作为研究对象,开展了CuO-ENPs在20、40 mg/L浓度下对三疣梭子蟹的慢性毒性试验,探讨了CuO-ENPs在不同组织内的积累效应。结果表明:CuO-ENPs的富集含量从高到低的顺序为鳃>血淋巴>肌肉>肝胰腺>心脏和胃,三疣梭子蟹暴露于不同CuO-ENPs浓度的条件下,体内CuO-ENPs的积累量具有一定差异,除血淋巴外,随着CuO-ENPs浓度的增高,不同组织的CuO-ENPs残留量有一定程度的增加,表现出明显的浓度效应。三疣梭子蟹鳃对CuO-ENPs的富集能力较强,在20、40 mg/L浓度下富集系数分别高达76.35和58.15,血淋巴对CuO-ENPs的富集系数分别为2.6和1.4,肌肉对CuO-ENPs的富集系数分别为1.77和1.43,肝胰腺对CuO-ENPs的富集系数分别为1.3和0.8,心脏和胃对CuO-ENPs的富集系数分别为1.30和0.65。三疣梭子蟹鳃对CuO-ENPs的富集作用远高于三疣梭子蟹的血淋巴、肌肉、肝胰腺和心脏及胃等组织。To study the accumulation effect of CuO-ENPs in different tissues of Portuns trituberculatus ,gills,haemolymph,muscles ,hepatop- ancreas, heart and the stomach of Portuns trituberculatus had been taken as objects in this study, and chronic toxicity test of Portuns trituberculatus had been carried out under the concentrations of CuO-ENPs of 20 mg/L and 40 mg/L, respectively.The results showed that the order from high to low of enrichment content of CuO-ENPs in different tissues of Portuns trituberculatus was gill〉haemolymph〉muscle〉hepatopancreas 〉heart and stomach. The enrichment content of CuO-ENPs in different tissues of Portuns trituberculatus was different with different concentration of CuO -ENPs.With the concentration of CuO-ENPs increased, the enrichment content of CuO-ENPs in different tissues was increased except for haemolymph.The enrichment ability of gills of Portuns trituberculatus was far higher than haemolymph,muscles,hepatopancreas,heart and the stomach.Under the concentrations of CuO-ENPs of 20 mg/L and 40 mg/L,respectively,the enrichment factors of gills were 76.35 and 58.15,respectively,while the enrichment factors of haemolymph were 2.6 and 1.4 ,respectively.The enrichment factors of muscles were 1.77 and 1.43 ,respectively.The enrichment factors of hepatopancreas were 1.3 and 0.8, respectively.The enrichment factors of heart and stomach were 1.30 and 0.65, respectively.

关 键 词：CuO-ENPs 三疣梭子蟹 积累 富集系数 

分 类 号：S968.252[农业科学—水产养殖]