五味子乙素对BaP致HTR-8/SVneo侵袭力损伤的保护  

The Protection Effect of Schisandrin B on Extravillous Trophoblast Cell Line Aggressive Damage Caused by BaP

作　　者：王梦[1] 梁婧[2] 侯海燕[1,3] 董渠龙 陈亚琼[1,3] WANG Meng LIANG Jing HOU Hai-yan DONG Qu-long CHEN Ya-qiong(Department of Obstetrics and Gynecology, Affiliated Hospital of Medical College of Chinese People＇s Armed Police Forces ,Tianjin 300162, China Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards , Tianjin 300162,China Guang＇anmen Hospital China Academy of Chinese Medical Sciences ,Beijing 100053, China)

机构地区：[1]中国人民武装警察部队后勤学院附属医院妇产科,天津300162 [2]中国中医科学院广安门医院南区 [3]天津市职业与环境危害防制重点实验室

出　　处：《国际妇产科学杂志》2016年第5期590-593,共4页Journal of International Obstetrics and Gynecology

基　　金：国家自然科学基金(81273977);武警后勤学院附属医院种子基金(FYM201415);天津市应用基础与前沿技术研究计划(15JCQNJC12300)

摘　　要：目的:以人绒毛膜外滋养层细胞(human trophoblast HTR-8/SVneo,简称HTR)细胞株为研究载体,研究五味子乙素(Schisandrin B,Sch B)对苯并芘(benzo[a]pyrene,BaP)致HTR细胞侵袭力损伤的影响。方法:实验分为对照组、BaP染毒组、不同浓度Sch B(0.5,1,2μmol/L)干预染毒组,通过新型四唑化合物(MTS)法检测细胞增殖情况,明确BaP染毒及Sch B干预剂量;通过Transwell侵袭实验检测BaP染毒后以及Sch B干预后细胞侵袭力的改变。结果:1MTS检测细胞增殖情况:与对照组相比,20μmol/L浓度BaP作用后细胞增殖明显下降(P<0.01),不同浓度Sch B(0.5,1,2μmol/L)干预后细胞增殖情况优于BaP染毒组(P<0.05)。2Transwell细胞侵袭实验:与对照组相比,BaP染毒组细胞侵袭力明显下降(P<0.01),不同浓度Sch B干预组细胞侵袭力也呈下降趋势(P<0.05);与BaP染毒组相比,Sch B干预组细胞侵袭力明显提升(P<0.05)。结论:一定浓度的BaP会对HTR的细胞增殖及侵袭力造成损伤,Sch B可以预防BaP对HTR细胞增殖造成的损伤,同时提高细胞侵袭力。Objective： Using human trophoblast HTR-8/SVneo （HTR） cell lines for the study of the carrier to study Schisandrin B （Sch B）′s action of benzo [a]pyrene （BaP） damage effect on HTR cell lines aggressivity. Methods：Experiment is divided into the control group, the BaP infected group, different dose Sch B （0.5, 1, 2 μmol/L）, intervention infected groupcell proliferation by MTS experimental detection, clear BaP canister and Sch B intervention dose, by Transwell invasion experiment after the detection of BaP infected cells and Sch B cell invasion force change after intervention. Results： ①The MTS test cell proliferation,compared with control group, 20 μmol/L BaP concentration cell proliferation significantly decreased after effect （P〈0.01）, after Sch B （0.5, 1, 2μmol/L） intervention the cell proliferation is obviously better than BaP infected group （P〈0.05）.②Transwell cell invasion experiment, compared with control group, the BaP cells infected group was much less aggressive （P〈0.05）. Sch B intervention group cell invasion force also declined （P〈0.05）. Compared with the infected group, Sch B cells of aggressive intervention group was obviously ascending （P〈0.05）. Conclusions：①A certain concentrations of BaP damaged HTR cell proliferation and invasion force. ②Sch B can prevent the damage of BaP of HTR cell proliferation, and at the same time improve cell aggressivity.

关 键 词：五味子属 苯并芘 细胞增殖 滋养层细胞 

分 类 号：R285[医药卫生—中药学]