一测多评法与外标法测定胆黄连中生物碱类成分含量比较  

Comparison of Simultaneous Determination of Alkaloids in Bile Processed Coptidis Rhizoma by QAMS and External Standard Method

作　　者：王静[1] 陈悦 袁子民[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]北京同仁堂科技发展股份有限公司,北京100079

出　　处：《中国中医药信息杂志》2016年第11期78-80,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81303225)

摘　　要：目的建立一测多评法(QAMS)测定胆黄连中3种生物碱成分含量,并与外标法测定结果进行比较,探讨QAMS的可行性。方法采用高效液相色谱法,以盐酸小檗碱为内参物,建立盐酸小檗碱与盐酸巴马汀、盐酸药根碱间的相对校正因子,用所得相对校正因子进行含量计算(计算值),实现一测多评。同时采用外标法测定3个成分的含量(实测值),比较计算值与实测值的差异。结果 10批胆黄连中3种生物碱成分含量的计算值与实测值无显著差异。结论 QAMS可用于胆黄连中生物碱类成分的含量测定。Objective To establish QAMS method to determine the contents of three alkaloids in bile processed Coptidis Rhizoma; To compare the results of QAMS with those from external standard method; To prove the feasibility of QAMS. Methods An HPLC method was developed. Berberine hydrochloride was selected as the internal reference substance. 2 relative correction factors（RCF） of berberine hydrochloride to palmatine hydrochloride and to jatrorrhizine hydrochloride were established. Obtained RCFs were used to conduct content calculation（calculated value） to complete QAMS method. At the same time, the contents（measured value） of the three components were also determined by external standard method. Calculated value and measured value were compared. Results The analysis results showed that there was no significant difference between the calculated values and the measured values of the three alkaloids in 10 batches of bile processed Coptidis Rhizoma. Conclusion The QAMS method can be applied in the determination of alkaloids in bile processed Coptidis Rhizoma.

关 键 词：一测多评法 外标法 胆黄连 生物碱 相对校正因子 

分 类 号：R284.1[医药卫生—中药学]