不同产地五味子药材色谱图谱研究  

Study on HPLC Fingerprints of Schisandrae Chinensis Fructus from Different Producing Areas

作　　者：王连芝[1] 付克[1] 顾媛媛[1] 刘磊[1] 张淑香[1] 周琦[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040

出　　处：《中国中医药信息杂志》2016年第11期103-106,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12531628)

摘　　要：目的采用高效液相色谱法建立五味子药材的指纹图谱研究方法,并对不同产地五味子指纹图谱进行相似度分析。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速1.0 m L/min,柱温30℃,检测波长254 nm。结果建立了五味子药材的高效液相色谱指纹图谱研究方法,确定了指纹图谱中29个共有峰,各产地药材指纹图谱的相似度均在0.95以上。结论本方法简便易行,可为评价五味子质量提供依据。Objective To establish the fingerprint analysis method of Schisandrae Chinensis Fructus by HPLC; To analyze the similarity on the fingerprints of Schisandrae Chinensis Fructus from different producing areas. Methods The chromatographic separation was performed by HPLC on a Diamonsil C18 column（250 mm×4.6 mm, 5 μm）. Acetonitrile-water was used as gradient mobile phase. The flow rate was 1.0 m L/min. The column temperature was maintained at 30 ℃. The detection wavelength was set at 254 nm. Results The HPLC fingerprint analysis method of Schisandrae Chinensis Fructus was established. Twenty-nine common fingerprint peaks were identified. The similarities of the fingerprints of ten samples from different producing areas were above 0.95. Conclusion The method is simple and reliable, which can provide a scientific basis for quality evaluation of Schisandrae Chinensis Fructus.

关 键 词：五味子 指纹图谱 高效液相色谱法 质量控制 

分 类 号：R284.1[医药卫生—中药学]