响应面法优化亚临界水萃取黑木耳多糖工艺  

Optimization of Subcritical Water Extraction of Polysaccharides from Auricularia auricular by Response Surface Methodology

作　　者：包怡红[1,2] 邓启[1] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]林下经济资源研发与利用协同创新中心,黑龙江哈尔滨150040

出　　处：《食品与生物技术学报》2016年第10期1053-1060,共8页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2012BAD34B04)

摘　　要：利用响应面法对亚临界水萃取法提取木耳多糖的工艺条件进行优化。以木耳粉末为材料,研究温度、时间、液料比、压力对木耳多糖提取率的影响,用苯酚-硫酸法检测木耳多糖的含量,基于单因素试验结果,采用Box-Behnken试验设计,最终确定木耳多糖的最佳提取工艺,并与其他提取方法进行比较。经过响应面试验优化,最佳提取条件为:温度152℃,时间26 min,液料比131∶1(m L/g),提取压力1.0 MPa,此条件下木耳多糖提取率为24.51%。利用亚临界水萃取法,木耳多糖提取率得到大幅提高,与传统的热水浸提法相比提高了3.82倍,为木耳多糖的工业化生产提供了一种高效环保技术。The optimization of subcritical water extraction of polysaccharides from Auricularia auricular was investigated by response surface methodology （RSM）. The effect of extraction condition on the yield of polysaccharies in Auricularia auricular powder was studied,including temperature,extraction duration,liquid-solid ratio and pressure. The content of polysaccharide was determined using phenol-sulfuric acid colorimetry. A Box-Behnken design was used to achieve the best extraction procedure based on the results of single-factor experiments. The comparison of different extraction methods was also done. The optimized extraction procedure was performed by 131∶1 of liquid-solid ratio at 152 ℃ for 26 min under the pressure of 1.0 MPa with the polysacchariesn yield of 24.51%. The yield of polysacchariesn was significantly increased using the proposed subcritical water extraction procedure,which was 3.82 fold of that extracted by traditional hot water method. This study provides a high-efficiency and eco-friendly method for industrial production of polysaccharides from Auricularia auricular.

关 键 词：黑木耳 多糖 亚临界水 响应面法 提取 

分 类 号：TQ281[化学工程—有机化工]