Neuropilin-1的干扰RNA对胃癌细胞株AGS生物学行为的影响  

Effect of siRNA-NRP-1 on biological behavior of gastric cancer cells line AGS

作　　者：方圆 徐卫国 齐艳涛 杨少君 徐洪 杨鑫 赵跃鹏 梁君伟[5] 李君辉[6] 刘和亮 

机构地区：[1]华北理工大学附属医院肿瘤外科,河北唐山063000 [2]山东万杰肿瘤医院放疗二科,山东淄博255200 [3]华北理工大学医学实验研究中心,河北唐山063000 [4]承德县医院普外科,河北承德067000 [5]承德医学院附属医院肿瘤科,河北承德067000 [6]唐山市第三医院肿瘤科,河北唐山063000

出　　处：《现代肿瘤医学》2016年第22期3539-3542,共4页Journal of Modern Oncology

基　　金：河北省科学技术研究与发展计划项目(编号:10276115D)

摘　　要：目的:探讨神经纤毛蛋白-1(neuropilin-1,NRP-1)的小干扰RNA(small interfering RNA,siRNA)对人胃腺癌细胞株AGS某些生物学行为的影响。方法:通过脂质体lip-2000分别将NRP-1特异性siRNA、control siRNA转染入人胃腺癌AGS细胞株作为转染组、无意义序列组,加入PBS的细胞作为空白对照组,采用MTT法检测三组细胞24h、48h、72h的增殖情况;Transwell小室侵袭和迁徙实验检测细胞的迁徙和侵袭能力。结果:转染后,转染组细胞的增殖能力在24h、48h和72h与对照组相比,均显著下降(P=0.00)。小室迁徙及侵袭实验表明:与对照组相比,转染组的胃癌AGS细胞迁徙能力显著下降,差异具有统计学意义(P<0.05)。转染组的胃癌AGS细胞侵袭能力显著下降,差异具有统计学意义(P<0.05)。结论:Neuropilin-1的siRNA能够明显影响人胃癌细胞株AGS的某些生物学行为。Objective： To obverse the effect of siRNA- NRP- 1 transfected gastric cancer cells AGS. Methods：NRP1- specific small interfering plasmid,siRNA- NRP- 1 and control siRNA- NRP- 1 were constructed and transfected to gastric cancer cells AGS in mediation of liposome. Respectively as an experimental group and a control group. The cells after adding PBS as a blank group. The expression of the proliferation level of AGS cells by MTT method respectively in 24,48,72 hours. Transwell chambers experiments detected the migration and invasion ability of the three groups. Results： The cells ＇ proliferation of experimental group was significantly less than control groups at24,48,72 hours（ P = 0. 00）. Transwell chambers experiments showed thatthe transfection group compared with control groups,a significant reduction in the invasive ability,statistically significant difference（ P〈 0. 05）,migratory ability decreased significantly,the difference statistically significant（ P〈 0. 05）. Conclusion： siRNA- NRP- 1 can influence the biological behavior of gastric cancer cells AGS clearly.

关 键 词：神经纤毛蛋白-1 人胃癌细胞株AGS siRNA 细胞增殖 Transwell小室实验 

分 类 号：R735.2[医药卫生—肿瘤]