中国水仙类黄酮-3-O-葡糖基转移酶基因的原核表达  

Prokaryotic Expression of 3 GT Gene from Narcissus tazetta var. Chinensis

作　　者：王伟英[1] 李海明[1] 戴艺民[1] 李跃森[1] 邹晖[1] 吴水金[1] 林江波[1] 

机构地区：[1]福建省农业科学院亚热带农业研究所,福建漳州363005

出　　处：《福建农业学报》2016年第8期816-819,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2014J01098)

摘　　要：通过双酶切、连接转化等方法将中国水仙类黄酮-3-O-葡糖基转移酶(NT3GT)基因克隆到原核表达载体pET29a上,构建原核表达重组质粒pET29a-NT3GT。重组质粒转化至E.coli BL21(DE3)后经IPTG诱导,SDS-PAGE分析表明,经IPTG诱导,NT3GT基因在大肠杆菌BL21(DE3)中获得了高效表达,表达的融合蛋白分子量约为55kDa。成功构建其原核表达载体并使其在大肠杆菌中得到高效表达,为NT3GT抗血清的制备及功能分析奠定基础。Flavonoid 3-O-glucosyltransferase（Nt3GT）gene of Narcissus tazetta var.Chinensis was cloned into the vector,pET29 a,to construct prokaryotic expression recombinant plasmid,pET29a-NT3 GT.The recombinant plasmid was transferred to E.coli BL21（DE3）to be induced by IPTG.Subsequently,the expression of the target protein was verified by SDS-PAGE.Then,pET29a-Nt3 GT was successfully constructed,which induced the 55 kDa recombinant protein of Nt3 GT in the prokaryotic expression system.Nt3 GT was cloned,and its vector was constructed and induced to be expressed successfully by IPTG in BL21 providing a basis for theantiserum preparation and functional analysis in the future.

关 键 词：中国水仙 类黄酮-3-氧-葡糖基转移酶 原核表达 

分 类 号：Q522[生物学—生物化学]