抗α-胞衬蛋白抗体对原发性干燥综合征的诊断价值  

The diagnostic value of anti-alpha cytoplasmic antibody in primary dry syndrome

作　　者：严鸣光[1] 方晓[1] 郭建军[1] 殷卫兵[1] 

机构地区：[1]商丘市第一人民医院检验科,河南商丘476100

出　　处：《现代仪器与医疗》2016年第5期84-86,共3页Modern Instruments & Medical Treatment

摘　　要：目的 :分析抗α-胞衬蛋白(α-Fodrin)抗体对原发性干燥综合征(Primary Sj?gren syndrom,pSS)的诊断价值。方法 :选取我院2012年9月—2015年9月收治的89例pSS患者,并选取同期45例继发性干燥综合征(Secondary SS,s SS)患者、91例系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者、154例类风湿性关节炎(Rheumatoid arthritis,RA)患者及100例健康体检者,使用酶联免疫吸附(ELISA)法对其血清抗α-Fodrin IgA、IgG抗体进行定量检测,均以检测结果 >15 U/m L为阳性标准,比较各组受试者血清α-Fodrin IgA、IgG抗体阳性率,并计算抗α-Fodrin抗体对pSS诊断的灵敏度、特异性,分析其诊断价值。结果 :pSS组抗α-Fodrin IgA、IgG抗体阳性率均显著高于其他各组,s SS组抗α-Fodrin IgA、IgG抗体阳性率均显著高于SLE组、RA组及对照组,差异有统计学意义(P<0.05);抗α-Fodrin IgA抗体诊断pSS的灵敏度、特异性分别为65.17%、94.62%,抗α-Fodrin IgG抗体诊断pSS的灵敏度、特异性分别为64.38%、96.67%。结论:抗α-Fodrin抗体诊断pSS有着较高的特异性,但灵敏度有限,故抗α-Fodrin抗体阳性仅可用于pSS的临床辅助诊断,但抗α-Fodrin抗体阴性患者仍不能除外pSS,需以病理检查明确诊断。Objective: To analyze the diagnostic value of anti-alpha-fodrin(α-fodrin) antibodies in primary Sj?gren's syndrome(pSS). Methods: 89 cases of pSS patients in our hospital from September 2012 to September 2015 were selected, and 45 cases of secondary Sjogren syndrome(secondary SS, s SS), 91 cases of patients with systemic lupus erythematosus(SLE), 154 cases of patients with rheumatoid arthritis(RA) and 100 healthy subjects during the same period were also included. Using enzyme-linked immunosorbent assay(ELISA) on the quantitative detection of serum anti-alpha-fodrin IgG and IgA antibodies, with the detection result of >15 U/m L as positive standard, compare the positive rate of serum anti-alpha-fodrin IgG and IgA antibodies in each group of subjects, and calculate and analyze the sensitivity and specificity of anti-alpha-fodrin antibodies on the diagnosis of pSS. Results: Positive rates of anti-alphafodrin IgG and IgA antibodies in pSS group were significantly higher than those in the other groups. Positive rates of anti-alpha-fodrin IgG and IgA antibodies in s SS group were significantly higher than those in SLE group, RA group and control group, the differences were statistically significant(P<0.05); sensitivity and specificity of anti-alpha-fodrin IgA antibodies in the diagnosis of pSS were 65.17% and 94.62%, and sensitivity and specificity of anti-alpha-fodrin IgG antibodies in the diagnosis of pSS were 64.38% and 96.67%, respectively. Conclusions: anti-alpha-fodrin antibodies in the diagnosis of pSS with high specificity, but the sensitivity is limited, so anti-alpha-fodrin antibodies which were positive can only used for clinical assistant diagnosis of pSS, but the anti-alpha-fodrin antibodies negative patients still cannot be excluded with pSS, which should be pathologically examined for clear diagnosis.

关 键 词：抗Α-胞衬蛋白抗体 原发性干燥综合征 诊断 

分 类 号：R392[医药卫生—免疫学]