培养基中几种重要营养元素对Anabaena sp.strain PCC 7120及Synechocystis sp.strain PCC 6803生长的影响  

Effects of important nutrition elements in medium on the growth of Anabaena sp.strain PCC 7120 and Synechocystis sp.strain PCC 6803

作　　者：高良春 张巨源[1] 王莉[1] 陈雯莉[1] GAO Liangchun ZHANG Juyuan WANG Li CHEN Wenli(State Key Laboratory of Agricultural Microbiology , Huazhong Agricultural University ,Wuhan 430070, Chin)

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2016年第6期17-24,共8页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31570048);中央高校基本科研业务费专项(2014PY003)

摘　　要：配制KNO3、KHCO3、NaHCO3、FeNa2-EDTA及Na2S2O3营养盐培养液,并按一定浓度添加至液体或固体培养基中,获得含有不同营养素、不同浓度的液体及固体培养基,检测无机氮(NO-3)、无机碳(HCO-3)、Fe及Na2S2O3对集胞蓝细菌Synechocystis sp.strain PCC 6803和鱼腥蓝细菌Anabaenasp.strain PCC 7120细胞生长的影响。结果显示,集胞蓝细菌Synechocystis sp.strain PCC 6803在10mmol/L硝酸盐生长较好,鱼腥蓝细菌Anabaena sp.strain PCC 7120在2mmol/L硝酸盐生长较好。不同浓度KHCO3及NaHCO3都会对蓝细菌的生长产生影响,可能是因为盐离子发挥了主要作用。此外,2种蓝细菌在10mmol/L Na2S2O3中生长较好,不同浓度的铁盐对蓝细菌的生长产生较大影响,在10μmol/L铁盐中生长最佳。The cyanobacteria of Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120 are important models in basic and applied studies.Both species are conventionally cultured in BG11 , although they show very distinct physiology. The effects of several nutrients in BG11 medium on the growth of Anabaena sp. strain PCC 7120 and Synechocystis sp. strain PCC 6803 were investigated.Results showed that the growth of Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120 was best at 10 mmol/L NO3 and 2 mmol/L NO3 , respectively.Different concentrations of KHCO3 and NaHCOs Had different effects on the growth, indicating that both metal ions and HCO3 had a major role.For Na2S2O3 and Fe？the growth of Anabaena sp. strain PCC 7120 and Synechocystis sp. strain PCC 6803 was best at 10 mmol/L and 10 μmol/L, respectively.

关 键 词：蓝细菌 培养基 营养元素 鱼腥蓝细菌 集胞蓝细菌 

分 类 号：Q93-335[生物学—微生物学]