黔产铁皮石斛对阿司匹林诱导胃黏膜上皮细胞GES-1损伤的保护作用研究  

Study on the protect effect of Dendrobium officinale Kimura et Migo from Guizhou on human gastric epithelial cell injury induced by aspirin

作　　者：杨传玉[1,2] 胡珺[1,2] 谭丹[1,2] 陈思颖[1,3] 杨畅[1,3] 孙佳[1,3] 刘亭[1,3] 王爱民[1,2] 

机构地区：[1]贵州医科大学药学院,贵州贵阳550004 [2]民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [3]贵州省药物制剂重点实验室,贵州贵阳550004

出　　处：《贵州医药》2016年第9期905-907,共3页Guizhou Medical Journal

基　　金：贵州省中药现代化科技产业研究开发专项项目[黔科合SY字(2014)3032-2号];民族药与中药开发应用产学研基地建设[黔科合KY字(2013)122];民族药化学成分及活性研究[黔科合人字(2015)11号)

摘　　要：目的探讨铁皮石斛全提物对阿司匹林诱导人胃黏膜上皮细胞GES-1细胞损伤的药效作用。方法体外培养人胃黏膜上皮细胞株GES-1细胞,经阿司匹林处理后,建立阿司匹林诱导GES-1细胞损伤模型;以阿司匹林联合铁皮石斛全提物各浓度预处理GES-1细胞为铁皮石斛组,以阿司匹林联合奥美拉唑预处理细胞为阳性组,并设立阴性对照组,采用MTS法检测细胞存活率,并通过比色法测定细胞上清液中LDH含量。结果当阿司匹林的浓度为18.5mmol/L处理GES-1细胞24h时,细胞的抑制率为40%;铁皮石斛浓度范围0~500μg/mL内,受损细胞存活率随铁皮石斛剂量增加而升高,LDH释放量逐渐降低（P〈0.05）。结论铁皮石斛对于阿司匹林诱导GES-1细胞损伤具有保护作用。Objective To investigate the protective effects of Dendrobium officinale Kimura et Migo extracts on aspirin-induced injury in human gastric mucosal epithelium cells （GES-1）. Methods Human gastric epithelial cell line GES-I was cultured in vitro. In aspirin group, GES-t cells were treated with aspirin. GES-1 cells in Dendrobium officinale Kimura et Migo group were pretreated with different concentrations of Dendrobium officinale Kimura et Migo extracts before aspirin treatment. In omeprazole group, GES-1 cells were pretreated with omeprazole before aspirin treatment. And a negative control group was established simultaneously. MTS assay was used to detect the cell viability. The content of LDH was also determined by the LDH kit. Results Incubated at 18.5 mmol/L of aspirin for 24 h, GES-1 cell inhibitory rate reached 40% as the injury model of GES-1 cells, 0~500 μg/mL concentrations of Dendrobium officinale Kimura et Migo had a protective effect on the GES-1 ceils to some extent, respectively. Mo- reover, these protective effects were more obvious with the increase of concentrations of Dendrobium officinale Kimura et Migo extracts. And the content of LDH was also released. Conclusion Dendrobium officinale Kimura et Migo extracts can promote proliferation to injury human gastric epithelial cell line GES-1 induced by aspirin.

关 键 词：铁皮石斛 GES-1细胞 阿司匹林 MTS方法 LDH含量测定 

分 类 号：R285.5[医药卫生—中药学]