早期糖尿病肾病大鼠肾脏线粒体Ca^(2+)及细胞色素C变化  

Changes of Renal Mitochondrial calcium and cytochrome C levels in Early Diabetic Nephropathy Rats

作　　者：杨前勇[1] 张静[2] 聂忠[1] 李刚[2] 熊亚丹[1] 

机构地区：[1]中国人民解放军第94医院内分泌科,江西南昌330002 [2]中国人民解放军第94医院药学科,江西南昌330002

出　　处：《贵州医药》2016年第9期911-912,共2页Guizhou Medical Journal

基　　金：南京军区医药卫生课题重点项目(11z018)

摘　　要：目的观察早期糖尿病肾病大鼠肾脏线粒体Ca2+及细胞色素C(Cyt C)的变化。方法将20只Wistar大鼠随机分为对照组、模型组,每组10只。模型组建立链脲佐菌素诱导早期糖尿病肾病大鼠模型,对照组大鼠未经链脲佐菌素诱导。第10周末测定两组血糖、肾质量指数、24h尿蛋白定量;检测两组大鼠肾脏线粒体Ca2+、Cyt C含量。结果与对照组相比,模型组大鼠的血糖、肾质量指数、24h尿蛋白定量均显著升高(均P<0.01),线粒体Ca2+及胞浆Cyt C含量升高,线粒体Cyt C含量显著降低(P<0.05)。结论早期糖尿病肾病大鼠肾脏线粒体内钙超载,线粒体Cyt C释放增加。Objective To study the changes of renal mitochondrial calcium and cytochrome C levels in rats with early diabetic nephropathy（DN）. Methods Model group was induced by injection of streptozotocin. Twenty male Wistar rats were divided into as control group and model group, with ten rats in each group. Ten weeks later, blood glucose level, renal mass index, 24 hour urinary protein excretion and levels of renal mitochondrial calcium and cytochrome C were measured. Results Compared with control group, Blood glucose level, renal mass index,24 hour urinary protein excretion , renal mitoehondrial calcium level were significantly increased （all P〈0.01） , cytoplasm cytochrome C levels increased and renal mitochondrial eytochrome C levels decreased in model group （P〈0.05）. Conclusion This study suggested mitochondrial calcium overload and the release of mitochondriat cytochrome C were elevated in early DN rats.

关 键 词：糖尿病肾病 线粒体 线粒体钙 细胞色素C 大鼠 

分 类 号：R654.2[医药卫生—外科学]