二乙基对硝基苯磷酯酶2基因G/A148多态性与2型糖尿病及血脂的关系  

An association study of paraoxonase gene G/A148 polymorphism with type 2 diabtets mellitus and blood lipids level in Chinese population

作　　者：冯雁[1] 李琼芳[1] 钱荣立[2] 洪天配[1] 关宝祥[3] 陈常中[4] 王朝曦[4] 徐肇翊 徐希平[3] 

机构地区：[1]北京大学第三医院内分泌科,100083 [2]北京大学第一医院内分泌科 [3]北京大学医学部生态遗传和生殖卫生研究中心 [4]哈佛大学公共卫生学院 [5]辽宁省卫生防疫站

出　　处：《中国糖尿病杂志》2002年第4期195-198,共4页Chinese Journal of Diabetes

摘　　要：目的　了解二乙基对硝基苯磷酯酶 (PON) 2基因 G/A14 8多态性与中国北方地区人群2型糖尿病的相关性及其与血脂等的关系。　方法　采用配偶对的病例 -对照研究设计 ,用聚合酶链反应 (PCR) -长度多态性的方法检测 PON2基因 G/A14 8多态性。　结果　 PON2基因 G/A14 8多态性的基因型频率和等位基因频率在 2型糖尿病组和对照组间差异无显著性意义。与非肥胖、无 G等位基因相比 ,肥胖无 G等位基因时 ,发生 2型糖尿病的危险度 (OR)值为 2 .0 4 ;肥胖有 G等位基因时 ,OR增至 3.18。在对照人群中 ,含 G等位基因组的总胆固醇 (TC)、低密度脂蛋白 (L DL)及载脂蛋白 B(Apo B)水平均比 AA基因型组高。G等位基因对高脂血症的 OR为 2 .4 8,P=0 .0 5。　结论　在中国北方地区人群中 PON2基因 G/A14 8多态性与肥胖有协同促进 2型糖尿病发生的作用。在非糖尿病人群中 。Objective To test the role of paraoxonase (PON) 2 gene in the pathogenesis of type 2 diabetes mellitus in Chinese population. Methods The PON2 gene G/A148 polymorphism was detected by polymerase chain reaction (PCR) and appropriate restriction enzyme (PCR RFLP) in 132 couples of type 2 diabetes spouse based controls. Results The genotype frequency and allele frequency were not different between the case and the control group. Compared with non obesity subjects without G allele, the obesity subjects without G allele and the obesity subjects with G allele had an OR for type 2 diabetes of 2.04, 3.18, respectively. In control group, the levels of total and low density lipoprotein cholesterol and apolipoprotein B (ApoB) were higher in the GA/GG genotype than that in the AA genotype and the OR of G allele for hyperlipidemia was 2.48, P =0.05. Conclusion There were synergistic effects between the PON2 gene G allele and obesity on type 2 diabetes mellitus. PON2 gene G/A148 polymorphism was significantly associated with total cholesterol level in non diabetic Chinese population.

关 键 词：2型糖尿病 二乙基对硝基苯磷酯酶2基因 血脂 基因多态性 聚合酶链反应-长度多态性 

分 类 号：R587.1[医药卫生—内分泌]