复方竹叶石膏颗粒对急性放射性食管损伤家兔免疫功能的调节作用  

Immunostimulatory Effect of a Compound of Zhuye Shigao Oral Granule on Radiation-induced Esophagitis

作　　者：王丽娟[1] 路军章[1] 李明伟[1] 牛宝龙[1] 曲宝林[1] WANG Lijuan LU Junzhang Ll Mingwei et al(China Shigao Oral Granule on Radiation-induced Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]解放军总医院,北京100853

出　　处：《中国中医急症》2016年第10期1855-1858,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81357553)

摘　　要：目的观察复方竹叶石膏颗粒对急性放射性食管损伤家兔免疫功能的调节作用。方法 40只清洁级雄性家兔随机分为空白对照组、单纯照射组(模型组)、复方竹叶石膏组、康复新液组,每组10只,除空白对照组以外,其余组家兔均以直线加速器6MV-X线一次照射32 Gy,建立家兔急性放射性食管损伤模型,各组均按不同治疗方法于照射前1周灌胃至照射完成后1周结束,每日1次。用流式细胞仪检测CD4^+、CD8^+百分比,用免疫浊度法检测IgG及补体C3的含量。结果放射线导致急性放射性食管损伤家兔血中CD4^+与CD8^+的百分比、CD4^+/CD8^+值和IgG与补体C3的水平降低;与模型组比较,药物组提高家兔外周血CD4^+、CD4^+/CD8^+、IgG及补体C3的值(P<0.05);与康复新组比较,复方竹叶石膏颗粒组在提高IgG与补体C3水平效果更为显著(P<0.05),外周血CD4^+、CD8^+、CD4^+/CD8^+比较无统计学差异(P>0.05)。结论复方竹叶石膏颗粒对急性放射性食管损伤家兔的免疫功能具有显著修复作用。Objective： To study the immune repairing effect of a compound Zhuye Shigao Granule （CZYG） in treating rabbit radiation-induced esophageal injury. Methods： Forty rabbits, clean grade, male, were randomly divided into the blank control group, simple irradiation group （model group）, CZYG group, and Kangfu Xinye group, irradiated with a linear accelerator of 6MV X ray at a dose of 32 Gy,except the blank control group,to establish the model of acute radiation-induced esophagitis （RE）. Each group was treated with different drugs and started from one week before the irradiation to one week after the completion of the irradiation, once a day. The percent- age of CD4＋ and CD8＋ were detected by flow cytometry,and the content of IgG and complement C3 were detected by immune turbidity method. Results： The percentage of CD4＋, CD8＋, the value of CD4＋/CD8＋ and the level of IgG and complement C3 decreased in the model group. The percentage of CD4＋,CD8＋ ,the value of CD4＋/CD8＋ in the Peripheral Blood and the level of IgG and complement C3 were improved in the drug groups compared with model group（P〈 0.05）, and the level of IgG and complement C3 were more remarkable in the CZYG than Kangfu Xinye group （P〈 0.05）. The comparison on the value of CD4＋, CD8＋ and CD4＋/CD8＋ in the Peripheral Blood had no statistical difference （P〉 0.05）. Conclusion： This study suggests that CZYG is able to repair the impaired cellular and humoral immunity of rabbits with RE.

关 键 词：急性放射性食管炎(RE) 复方竹叶石膏颗粒 免疫功能 

分 类 号：R285.5[医药卫生—中药学]