刘豨逍遥五苓汤对肝纤维化大鼠病理组织学及超微结构的影响  

Effect of Liuxi Xiaoyao Wuling Decoction on Histopathology and Ultrastructure of Hepatic Fibrosis in Rats

作　　者：姜一鸣[1] 姜学连[1] 刘新军[1] 孙建[1] 王慧凯[1] 路玉良[1] 宋荣强[1] 魏铭[1] JIANG Yiming JIANG Xuelian LIU Xinjun et al(Affiliated Hospital of Binzhou Medical College, Shandong,Binzhou 256600,Chin)

机构地区：[1]滨州医学院附属医院,山东滨州256600

出　　处：《中国中医急症》2016年第10期1877-1880,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划(2009-185)

摘　　要：目的观察刘豨逍遥五苓汤对肝纤维化大鼠的组织学及超微结构变化的影响。方法用腹腔注射猪血清法制作免疫性大鼠肝纤维化模型,用刘豨逍遥五苓汤进行预防和治疗,并与秋水仙碱对照,用光镜观察肝脏组织学染色(HE染色)、Masson三色胶原纤维染色的变化,用电镜观察肝脏组织超微结构的变化。结果中药治疗组肝脏HE染色、Masson染色及超微结构的改善情况明显优于西药对照组(P<0.01)。结论刘豨逍遥五苓汤能够保护肝细胞,减轻肝细胞变性、坏死,抑制贮脂细胞的活化和增殖,减少胶原纤维的成和分泌,阻抑肝纤维化发展。Objective： To observe the changes of histopathology and uhrastructure of Liuxi Xiaoyao Wuling Decoction on hepatic fibrosis in rats. Methods： The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum,and treated and prevented with Liuxi Xiaoyao Wuling Decoction,and compared with colchicine. The changes of HE and Masson of the rats＇ liver were observed with optic mi- croscope;the ultrastructure was observed with electronic microscope. Results： The improvement of HE staining,Masson staining and uhrastructure of the treatment group was superior to that of the control group obviously （P〈0.01）. Conclusion： Liuxi Xiaoyao Wuling Decoction can protect liver cells,reduce liver cell degeneration and necrosis,inhibit fat storing cell＇s activation and proliferation,reduce collagen fiber formation and secretion ,and inhibit the development of liver fibrosis.

关 键 词：刘豨逍遥五苓汤 肝纤维化 组织学 超微结构 

分 类 号：R285.5[医药卫生—中药学]