胞红蛋白对巨噬细胞氧化损伤的保护作用  

Protective effect of CYGB on Macrophage during Oxidative Damage

作　　者：屈顺林[1] 朱宇凝 潘文军[1] 李维[1] 王宇菲[1] 谢黎明[2] 姜志胜[1] 范文静[3] 

机构地区：[1]南华大学医学院病生教研室心血管病研究所动脉硬化学湖南省重点实验室,湖南衡阳421001 [2]南华大学附属第一医院 [3]南华大学附属第二医院急诊科

出　　处：《中南医学科学杂志》2016年第5期486-489,共4页Medical Science Journal of Central South China

基　　金：国家自然基金项目(编号:81670424);国家安全生产重大事故防治关键技术科技项目(hunan-0013-2016AQ);湖南省科技厅项目(编号:2015SK20203;2011TT2051);湖南自然科学基金项目(编号:2015JJ2118);湖南省教育厅重点课题(编号:2015JJ2118);湖南省卫计委科研项目(编号:B2016087);湖南省研究生科研创新项目(CX2016B480);中国博士点基金项目(编号:20114324120004)

摘　　要：目的探讨CYGB在巨噬细胞氧化损伤过程中的表达及作用。方法构建CYGB高表达/低表达巨噬细胞模型,Western blot检测不同浓度H_2O_2刺激RAW264.7 12 h后CYGB的蛋白表达水平;用分光光度计观察RAW264.7在氧化损伤过程中乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量的变化情况。结果 0.5 mmol/L H_2O_2处理巨噬细胞12 h后CYGB表达水平明显高于空白对照组(P<0.05);与0.5 mmol/L H_2O_2处理组相比,0.5 mmol/L H_2O_2对CYGB高表达组的CYGB表达升高(P<0.05),而0.5 mmol/L H_2O_2对CYGB低表达组的CYGB表达降低,差异有统计学意义(P<0.05);0.5 mmol/L H_2O_2+CYGB高表达组中LDH活性和MDA含量下降;0.5 mmol/L H_2O_2+CYGB低表达组中LDH活性和MDA含量升高,差异有统计学意义(P<0.05)。结论巨噬细胞氧化损伤时CYGB表达水平增加;CYGB在巨噬细胞氧化损伤过程中具有拮抗作用。Objective To observe change of cytoglobin（ CYGB） expression and explore the protective role of CYGB during oxidative stress in macrophages. Methods Western blot was used to detect CYGB expression after 0. 5 mmol/L H2O2 treatment for 12 h.Cell model of CYGB overexpression and downexpression was constructed,then to observe the role of CYGB during oxidative stress.After treatment with H2O2 for 12 h,CYGB protein levels,lactate dehydrogenase（ LDH） and malondialdehyde（ MDA） were detected in each group. Result The expression of CYGB in H2O2-treated group was increased compared with control group,the difference was significant（ P 0. 05）. The expression of CYGB was higher in0.5 mmol/L H2O2treatment＋CYGB expression group and lower in 0.5 mmol/L H2O2treatment＋CYGB interference group compared with the 0.5 mmol/L H2O2 treated group（ P〈0.05）.Results showed that LDH,MDA level was decreased in H2O2treatment＋CYGB expression group and increased in H2O2treatment＋CYGB interference group. Conclusion Expression of CYGB in macrophage was increased during oxidative stress.The CYGB protected against oxidative injury in macrophage.

关 键 词：巨噬细胞 氧化应激 胞红蛋白 过氧化氢 

分 类 号：R36[医药卫生—病理学]