梅毒螺旋体膜脂蛋白Tp0821促炎活性研究  

Proinflammatory Effect of Treponema Pallidum Membrane Lipoprotein Tp0821

作　　者：吴志周[1] 文美贞[1] 柯建良[1] 祝新[1] 谭雪玲[1] 

机构地区：[1]江门市皮肤医院检验科,广东江门529000

出　　处：《中南医学科学杂志》2016年第5期515-518,共4页Medical Science Journal of Central South China

摘　　要：目的探讨梅毒螺旋体(Tp)膜脂蛋白Tp0821潜在的促炎活性及可能参与的信号转导通路。方法表达重组蛋白Tp0821(r Tp0821),去除其中内毒素。r Tp0821刺激THP-1细胞,ELISA检测促炎细胞因子IL-6、IL-8和IL-1β的表达水平;ERK1/2、JNK、p38和NF-κB特异性抑制剂分别预处理THP-1细胞30 min,再以r Tp0821刺激THP-1细胞,ELISA分别检测IL-6、IL-8和IL-1β的表达水平。结果 r Tp0821刺激THP-1细胞表达IL-6、IL-8和IL-1β在一定范围内与刺激时间以及蛋白浓度呈正相关,1.0μg/m L刺激24 h时各细胞因子表达水平最高;r Tp0821刺激经ERK1/2、JNK、p38和NF-κB特异性抑制剂预处理后的THP-1细胞,IL-6表达水平分别下降63.4%(P<0.001)、4.3%(P>0.05)、53.4%(P<0.001)和73.1%(P<0.0001),IL-8分别下降49.2%(P<0.001)、6.0%(P>0.05)、41.1%(P<0.001)和71.0%(P<0.001),IL-1β分别下降36.8%(P<0.01)、3.9%(P>0.05)、30.3%(P<0.001)和59.7%(P<0.001)。结论 r Tp0821可通过ERK1/2、p38和NF-κB通路诱导THP-1细胞表达促炎细胞因子IL-6、IL-8和IL-1β,Tp0821可能是Tp重要的致病因子。Objective To investigate the proinflammatory effect of Treponema pallidum membrane lipoprotein Tp0821 and related signal transduction pathways and provide experimental basis for the clarification of pathogenesis mechanisms of T. pallidum. Methods The recombinant protein Tp0821（ r Tp0821） was expressed and the endotoxin in r Tp0821 was removed.ELISA was used to detect the expression of proinflammatory cytokines IL-6,IL-8,and IL-1β of human THP-1 cells stimulated with r Tp0821. Furthermore,r Tp0821 was used to stimulate human THP-1 cells pretreated with the ERK1/2 inhibitor PD98059,p38 inhibitor SB203580,and NF-κB inhibitor BAY11-7082,respectively,and then ELISA was performed for detection of the expression level of proinflammatory cytokines. Results r Tp0821 possesses the ability to induce THP-1 cells to secret IL-6,IL-8,and IL-1β in dose-and time-dependent manners.The expression level of proinflammatory cytokines peaked when THP-1 cells were stimulated with 1.0 μg/m L of r Tp0821 for 24 hours.Moreover,when pretreated with PD98059,SP600125,SB203580,and BAY11-7082,respectively,THP-1 cells decreases expression of IL-6 by 63. 4%（ P0. 001）,4.3%（ P〈0.05）,53.4%（ P〈0.0001）,and 73.1%（ P〈0.0001）,IL-8 by 49.2%（ P〈0.0001） 、6.0%（ P〈0.05） 、41. 1%（ P〈0.0001）,and 71.0%（ P〈0.0001）,IL-1β by 36.8%（ P〈0.01）,3.9%（ P〈0.05）,30.3%（ P〈0.0001）,and59.7%（ P〈0.0001）,respectively. Conclusion These data indicate that the r Tp0821 may activate the THP-1 cells to produce the proinflammatory cytokines via ERK1/2,P38,and NF-κB signal transduction pathways and membrane lipoprotein Tp0821 might be an important pathogenic factor.

关 键 词：梅毒螺旋体 膜脂蛋白 Tp0821 促炎细胞因子 信号转导通路 

分 类 号：R377.1[医药卫生—病原生物学]