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作 者:谷伟[1] 户国[1] 孙鹏[1] 白庆利[1] 王炳谦[1]
机构地区:[1]中国水产科学研究院黑龙江水产研究所淡水鱼类育种国家地方联合工程实验室,黑龙江哈尔滨150070
出 处:《水产学杂志》2016年第5期12-16,共5页Chinese Journal of Fisheries
基 金:公益性行业(农业)科研专项(201403012);农业部948引进项目(2014-Z57)
摘 要:选择健康的体质量约为336g的褐鳟Salmo trutta 20尾,雌雄各半,在常规环境中暂养观察10d,禁食12h后,尾静脉采血,利用BT-2100全自动血液分析仪测定16项血液生理值,利用DXC800型贝克曼全自动生化分析仪测定31项血清生化值,以建立2龄褐鳟的血液正常生理生化指标。结果表明:雌雄褐鳟血液生理指标差异不显著(P>0.05),但血液中Mg、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)和直接胆红素(DBIL)含量及白球比(A/G)显著差异(P<0.05),说明褐鳟生理指标不受性别影响,而某些生化指标却受性别的影响。The normal physiological(16 items)and biochemical(31 items)indices were determined in blood sampled from caudal veins of twenty healthy two-year old brown trout Salmo trutta with body weight of about 336g(ratio of male to female=1:1)cultured in conventional environment for 10 days by an automatic animal analyzer and by automatic Beckmann-DXC800 BT-2100 analyzer after the fish were fasted for 12 h. The results showed that there were no significant differences in blood physiological indices between male and female(P 0.05). However, significant differences in serum biochemical indices including levels of Mg, total protein(TP),albumin(ALB), globulin(GLB), and direct bilirubin(DBIL)and albumin/globulin(A/G)value were observed between male and female(P 0.05), suggesting that blood physiological indices between male and female be coincident basically, being not affected by gender. The male and female brown trout, however, had some different serum biochemical indices.
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