谷胱甘肽-S转移酶基因多态性对6月龄幼儿智力发育的判断价值  

Influence of the polymorphism of glutathione S-transferases on the intelligence development of six-month-old infants

作　　者：贾晓 张倩 孙忠清[2] 谢萌[3] 王炳玲[2] 逄淑涛 

机构地区：[1]青岛市黄岛区妇幼保健与计划生育服务中心儿童保健科,266400 [2]青岛市疾病预防控制中心环境食品卫生科,266033 [3]青岛大学公共卫生学院流行病与卫生统计教研室,266021 [4]青岛市中心血站,266071

出　　处：《中华诊断学电子杂志》2016年第3期198-202,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：国家自然科学基金(81372955);青岛市医疗卫生优秀人才培养项目

摘　　要：目的：探讨幼儿谷胱甘肽-S转移酶（ GST）基因多态性对6月龄幼儿智力发育的判断价值。方法利用559名幼儿分娩时的脐带血提取DNA，采用多重扩增技术测定谷胱甘肽-S-转移酶M1-02（ GSTM1-02）的基因型， SequenomMassArray 系统测定 GSTM3（ rs7483）和 GSTT2（ rs140195、rs2719）的基因型。使用盖泽尔发育诊断量表测定6月龄幼儿5个能区的发育情况。结果幼儿5个能区的发育商＜90的占2．3％～6．8％。 GSTM1-02的两种基因型分布相近，rs7483次要等位基因频率C＝25．3％，rs140195次要等位基因频率A＝35．2％，rs2719的次要等位基因频率T＝25．6％。多因素分析显示rs7483的CC纯合子表现出了与幼儿适应性行为、大运动行为和精细动作行为得分相关性， OR及其95％CI分别为10．51（1．31，84．41）、4．44（1．18，16．72）和3．61（1．02，12．74）。结论 GSTM3的rs7483位点多态性可能在6月龄时即可影响到幼儿的智力发育。Objective To explore the possible association between the polymorphism of glutathione S-transferases （ GST） and the intelligence development of six-month-old infants.Methods DNA was extracted from the cord blood, and the GSTM1-02 genotypes were measured by multiple polymerase chain reaction （PCR）,and the genotypes of GSTM3 （rs7483） and GSTT2（rs140195,rs2719） were measured by the SequenomMassArray system.Gesell development diagnostic scale was used to measure the development quotients （ DQs） of 5 domains.Results The percentage of DQs less than 90 of the 5 domains was from 2.3%to 6.8%.The valid and invalid genotypes of GSTM1-02 was almost in the same percentage.The minor allele frequency of rs7483,rs140195 and rs2719 were 25.3%,35.2%and 25.6%,respectively.Multivariate analysis showed that CC genotype indicated higher risk for lower DQs of adaptive,gross motor and fine motor behaviors compared to TT genotype with OR and 95%CI 10.51（1.31,84.41）,4.44（1.18,16.72）and 3.61 （1.02, 12.74）, respectively.Conclusion The polymorphism of rs7483 of GSTM3 may influence the intelligence development even when the infant is 6 months old.

关 键 词：谷胱甘肽-S转移酶 基因多态性 幼儿 幼儿发育 

分 类 号：R174[医药卫生—妇幼卫生保健]