X射线诱变选育嗜酸氧化亚铁硫杆菌及其对难处理金矿浸出的研究  

Study on breeding Acidithiobacillus ferrooxidans induced by X-ray irradiation and bioleaching of refractory gold ore

作　　者：剡倩 张苗苗[1] 郭晓鹏[1,2] 李文建[1] 陆栋[1] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院大学,北京100049

出　　处：《辐射研究与辐射工艺学报》2016年第5期40-46,共7页Journal of Radiation Research and Radiation Processing

基　　金：中国科学院西部之光人才培养计划项目(Y306010XB0)资助~~

摘　　要：为了探讨嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans,At.f)经X射线诱变后对难处理金矿的预氧化效果和金浸出率的影响,采用不同剂量的X射线诱变处理At.f,通过微生物学方法和相关指标的测定,获得优良的At.f菌株。结果显示,经X射线辐照至吸收剂量为450 Gy时,At.f对Fe的浸出率达50.24%,矿石预氧化效果比原始菌株提高44%,金浸出率可达86.68%,是直接氰化时金浸出率的2.1倍。X射线能使At.f发生明显变异,使诱变菌的氧化活性、对难处理金矿的预氧化效果和金浸出率均得到提高。To study the influence of Acidithiobacillus ferrooxidans（At.f） mutated by X-ray on the biooxidation and bioleaching of refractory gold ores, X-ray was used to irradiate At.f strains at different doses, and more efficient strains were screened by microbiology methods and the measurement of relevant indicators. Compared with the original strain, the bioleaching ratio of iron by the mutants after irradiation at 450 Gy was 50.24%, namely 44% increased, and the bioleaching ratio of gold reached 86.68%-two folds than gold direct cyanidation leaching. It is proved that X-ray irradiation is an outstanding way to breed mutants of high bioleaching ratio of iron and gold strains of At.f.

关 键 词：嗜酸氧化亚铁硫杆菌 X射线诱变 生物氧化 难处理金矿 

分 类 号：Q691.5[生物学—生物物理学] TL99[核科学技术—核技术及应用]