汉滩病毒感染SCID小鼠动物模型的建立  

Establish an animal model of SCID mice infected with Hantaan virus

作　　者：马瑞雪[1] 程林峰[1] 马铁军[1] 王敬增 应旗康[1] 张晓晓[1] 刘蓉蓉[1] 王芳[1] 张芳琳[1] 吴兴安[1] 

机构地区：[1]第四军医大学基础部微生物学教研室,陕西西安710032

出　　处：《热带医学杂志》2016年第9期1089-1091,1095,F0004,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(31470890;31270978);陕西省科技统筹创新工程计划项目(2013KTCL03-06);军队重点课题(BWS13G024)

摘　　要：目的通过检测汉滩病毒(HTNV)76-118株感染SCID小鼠组织中的特异性病毒抗原、核酸的分布以及病理学变化,以建立汉滩病毒感染动物的评价体系。方法取汉滩病毒76-118株病毒液通过肌肉注射接种SCID小鼠,接种3 d后,取小鼠的心、肝、脾、肺、肾、脑等组织,并分别利用ELISA法检测各组织中的病毒特异性抗原;利用q RT-PCR法检测各组织中的病毒RNA;利用HE染色法检测各组织中的病理学变化。结果SCID小鼠感染汉滩病毒后,用夹心ELISA法可在其心、肝、脾、肺、肾组织中检测到HTNV特异性抗原,平均P/N值分别为2.33、7.41、8.13、7.40、2.89,其中脾病毒抗原含量最高;以q RT-PCR法检测小鼠各脏器组织中的HTNV核酸,在实验组小鼠的心、肝、脾、肺、肾中均可检测到病毒核酸,与对照组相比,其相对量分别达到2^(2.11)、2^(6.23)、2^(8.21)、2^(5.26)、2^(3.79)倍,其中脾病毒核酸含量最多;HE染色法可见肝、脾、肺中伴有显著的病理学变化。结论成功检测了汉滩病毒感染SCID小鼠后组织中特异性抗原、核酸的分布以及病理学变化,从而为建立汉滩病毒感染动物的评价体系提供一种参考。Objective To establish a system to evaluate the animals infected with Hantaan virus, through the detection of the specific antigens, nucleic acids, and histopathology in the tissues of the SCID mice infected with Hantaan strain 76-118. Methods SCID mice were intramuscular infected with Hantaan virus. Three days after infection, Hantaan virus antigens in the tissues were detected by ELISA;nucleic acids in the tissues were detected by q RT-PCR and histopathological analysis was detected by hematoxylin and eosin（HE） staining. Results Three days after infection,viralantigens could be detected from the heart, liver, spleen, lung and kidney of SCID miceby ELISA method, with the mean P / N value of 2.33, 7.41, 8.13, 7.40 and 2.89 respectively.The result from q RT-PCR suggested that the Hantaan virus nucleic acids were detected in the heart, liver, spleen, lung and kidney of SCID mice in experimental group;compared with controls, the relative magnitudes reached to 2^2.11, 2^6.23, 2^8.21, 2^5.26, 2^3.79 times, respectively. The results from histopathological HE staining demonstrated that significant alterations were found in the liver, spleen and lung.Conclusion The results provided some information about the animals infected with Hantaan virus.

关 键 词：汉滩病毒 SCID小鼠 抗原 病理学变化 

分 类 号：R392-33[医药卫生—免疫学]