HTNV嵌合VLP促进小鼠骨髓细胞活化的实验研究  

Bone marrow cells activation by Hantaan virus like particle

作　　者：马铁军[1] 张晓晓[1] 刘蓉蓉[1] 应旗康[1] 马瑞雪[1] 王敬增 程林峰[1] 刘梓谕[1] 王芳[1] 吴兴安[1] 

机构地区：[1]第四军医大学基础部微生物学教研室,陕西西安710032

出　　处：《热带医学杂志》2016年第9期1092-1095,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(31470890;31270978);陕西省科技统筹创新工程计划项目(2013KTCL03-06)

摘　　要：目的探讨嵌合有免疫刺激分子CD40配体(CD40L)或粒细胞巨噬细胞集落刺激因子(GM-CSF)的汉滩病毒(HTNV)样颗粒(VLP)对小鼠骨髓细胞活化的影响,以确定嵌合汉滩病毒样颗粒的生物学活性。方法利用真核细胞瞬时表达VLP、VLP-CD40L、VLP-GM-CSF,纯化后与小鼠骨髓细胞共培养,通过流式细胞术检测小鼠骨髓细胞活化情况。结果三种汉滩病毒VLP均能促进小鼠骨髓细胞活化(P<0.05),其中嵌合有GM-CSF因子的VLP促进小鼠骨髓细胞活化更为明显(P<0.01)。结论真核细胞表达的三种汉滩病毒VLP均有一定的生物学活性,为进一步研究汉滩病毒VLP疫苗奠定基础。Objective To analyze the bone marrow cell activation by Hantaan virus like particles and Hantaan virus like particles（VLP）-CD40L or-GM-CSF fusion proteins. Methods Recombinant VLP, VLP-CD40L and VLP-GM-CSF proteins were expressed and purified from eukaryotic cells, and were used to culture mice bone marrow cells. The biological activities were determined by flow cytometry. Result All the VLPs can activate bone marrow cells, and the VLP-GM-CSF was more effective. Conclusion The VLPs expressed in eukaryotic cells could effectively activate bone marrow cells, and this research laid a foundation for further study of Hantaan virus like particles.

关 键 词：汉滩病毒 病毒样颗粒 CD40L GM-CSF 生物学活性 

分 类 号：R392.33[医药卫生—免疫学]