miR-200a在胆道闭锁患儿肝组织中的表达  

Expression of miR-200a in liver of biliary atresia patients

作　　者：陈奕豪[1] 杨丽媛[1] 刘海英[1] 彭小芳[1] 庞舒尹 陈峥嵘[1] 王凤华[1] 陈翊[1] 

机构地区：[1]广州医科大学附属广州市妇女儿童医疗中心,广东广州510623

出　　处：《热带医学杂志》2016年第9期1100-1103,F0002,共5页Journal of Tropical Medicine

基　　金：广东省科技厅社会发展领域项目(2014A020212520)

摘　　要：目的检测胆道闭锁(BA)患儿肝组织及血浆miR-200a的表达,探讨其与BA肝纤维化及肝脏炎症的关系。方法采用逆转录PCR检测26例BA组、10例正常组血浆标本miR-200a的表达,并分析其与生化指标的相关性;采用酪胺信号放大荧光原位杂交(TSA-FISH)检测14例BA及2例正常肝组织标本miR-200a的表达;根据Ishak系统对BA肝组织进行肝纤维化及炎症评分,分析肝组织miR-200a表达与肝纤维化及炎症的相关性,并分析BA血浆与肝组织miR-200a的相关性。结果BA组血浆miR-200a表达显著高于正常组,差异有统计学意义(P<0.01);BA组血浆miR-200a表达与TB、DB、ALP、γ-GT、ALT、AST没有相关性(P>0.05)。BA组肝组织miR-200a表达显著高于正常组,BA肝组织纤维化区miR-200a表达显著低于没有纤维化的肝实质区;miR-200a与BA肝纤维化进展正相关(P<0.05),而与肝脏炎症程度无关(P>0.05)。9例检测了血浆miR-200a的肝组织标本中有6例出现肝组织miR-200a表达越高,血浆miR-200a表达越高的情况。结论miR-200a可能通过上皮间质转化、Wnt/β-catenin和TGF-β信号通路调控BA肝纤维化,并可能成为预测BA肝纤维化程度的血浆标志物。Objective To detect the expression of miR-200a in liver tissues and plasma of biliary atresia（BA） patients and investigate its effect on hepatic fibrosis and hepatic inflammation of BA. Methods Expression of miR-200a in plasma of BA group and normal group were detected by reverse transcription PCR, and the correlation between miR-200a and blood biochemical parameters of BA group was analyzed. Next, expression of miR-200a in liver tissues of BA group and normal group were detected by tyramide signal amplification fluorescence in situ hybridization（TSA-FISH）. Hepatic fibrosis and inflammation scores of liver tissues of BA were graded according to Ishak system; the correlations between miR-200a and hepatic fibrosis and inflammation were analyzed. Simultaneously, the correlation between plasma miR-200 a expression and relevant liver tissue miR-200a expression of BA was analyzed. Results In plasma, expression of miR-200 a in BA group was significantly higher than that in normal group（P〈0.01）. Expression of miR-200a in plasma of BA was not correlated with TB, DB, ALP, γ-GT, ALT and AST（P〉0.05）. In liver tissues, expression of miR-200 a in BA were markedly higher than that in normal group, and expression of miR-200a in fibrosis areas of BA liver was markedly lower than that in the liver parenchyma areas without fibrosis. There was a positive correlation between miR-200a and hepatic fibrosis in BA（P 〈0.05）,and miR-200a was not correlated with hepatic inflammation（P 〉0.05）. 6 out of 9 cases of BA group showed the higher expression of miR-200a in liver tissues, the higher was the expression of miR-200 a in plasma. Conclusion Mi R-200a may play a regulative role in hepatic fibrosis of BA via epithelial-mesenchymal transition, Wnt / β-catenin and TGF-β signal pathways, and possibly become a biomarker for predicting the grade of hepatic fibrosis in BA.

关 键 词：胆道闭锁 MICRORNA 肝纤维化 

分 类 号：R657.4[医药卫生—外科学]