人胶质瘤U251细胞定向诱导分化为脂肪细胞  

Induction and differentiation of human glioma cell line U-251 into the adipocytes

作　　者：王景文[1,2] 杨勇[2] 张志坚[1] 陈波[2] 苏春香[2] 胡昌龙[2] 蔡慧敏[1] 李巧玉[2] WANG Jing-wen YANG Yong？ ZHANG Zhi-jian CHEN Bo SU Chun-xiang HU Chang-long CAI Hui-minI LI Qiao-yu(School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013 Department of Neurosurgery, the Afiliated People＇s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China)

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]江苏大学附属人民医院神经外科,江苏镇江212002

出　　处：《江苏大学学报（医学版）》2016年第1期1-4,共4页Journal of Jiangsu University：Medicine Edition

基　　金：镇江市社会发展项目(SH2011035;SH2013050);江苏大学医学临床科技发展基金资助项目(JLY20140015)

摘　　要：目的:探讨人胶质瘤U251细胞的干细胞特性及定向诱导分化为脂肪细胞的潜能。方法:采用免疫荧光染色检测U251细胞中干细胞标志物巢蛋白,人类婆罗双树样基因4(sal-like 4,SALL4),神经细胞黏附分子(neural cell adhesion molecule,NCAM),胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达。采用成脂培养基诱导U251细胞,以CCK-8实验描记细胞生长曲线;油红-O染色及免疫印迹法检测脂肪细胞标志蛋白的表达。结果:胶质瘤细胞U251中巢蛋白,SALL4,NCAM,GFAP呈阳性表达;成脂诱导培养基定向诱导后,U-251细胞内大量橙红色脂滴形成;脂肪酸合酶、过氧化物酶体增殖物受体γ(PPARγ)、CCAAT增强子蛋白-β(CEBP-β)、脂联素表达增高。结论:人胶质瘤U251细胞具备干细胞表型,可定向诱导分化为脂肪细胞。Objective： To investigate the stem cell characteristics of human glioma cell line U251 and its potential of differentiating into adipocytes. Methods： Immunofluorescence staining was used to detect the expression of stem cell marker nestin, sal-like 4 （SALL4） , neural cell adhesion molecule （ NCAM ）, glial fibrillary acidic protein （GFAP） in U251 cells. Growth curve of U251 cells after adipogenic induction was evaluated by CCK-8 method. After the induction, fat deposition was stained by Oil Red O staining and adi- pogenic marker expression was detected by Western blotting. Results： U251 cells showed immunofluores- cence positive staining of nestin, SALL4, NCAM, GFAP. After adipogenic induction, a large number of or- ange-red lipid droplets was seen in U-251 cells; Western blotting showed increased expression of adipocyte- specific proteins, such as fatty acid synthase, proliferator-activated receptor gamma（PPARγ） , CCAAT en- hancer-bingding protein-β（CEBP-β）, adiponectin. Conclusion： U251 cells had stem cell phenotype and can be into adipocytes induction.

关 键 词：胶质瘤细胞 干细胞特性 脂肪细胞 诱导分化 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]