肝细胞核因子-4α启动子基因多态性与江苏地区非肥胖2型糖尿病患者胰岛素分泌相关  

A polymorphism of the hepatocyte nuclear factor-4α gene is associated with insulin secretion in non-obese subjects with type 2 diabetes from Jiangsu province

作　　者：高毅娜[1,2] 胡越[1] 王涛涛[1] 韦青[1,3] 孙湘[1] 俞力[1] GAO Yi-na HU Yue WANG Tao-tao WEI Qing SUN Xiang YU Li(Department of Endocrinology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001 Department of Endocrinology Zhumadian City Center Hospital, Zhumadian Henan 463000 Library of Jiangsu University, Zhenjiang Jiangsu 212013,China)

机构地区：[1]江苏大学附属医院内分泌科,江苏镇江212001 [2]驻马店市中心医院内分泌科,河南驻马店463000 [3]江苏大学图书馆,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2016年第1期57-60,共4页Journal of Jiangsu University：Medicine Edition

基　　金：镇江市分子内分泌重点实验室资助项目(SS2009012);镇江市社会发展资助项目(SH2013070)

摘　　要：目的:研究肝细胞核因子-4α(hepatocyte nuclear factor-4α,HNF-4α)基因的P2启动子区域的2个单核苷酸多态性(SNP)位点rs1884614和rs2144908与2型糖尿病的相关性。方法:收集2型糖尿病患者328例和非糖尿病患者196例,应用Taq Man SNP Genotyping系统进行rs1884614和rs2144908的基因型多态性分析。并分析它们与患者有关临床代谢指标的关系。结果:两组的基因型分布及等位基因频率差异无统计学意义(P>0.05);但在非肥胖(BMI<25 kg/m^2)患者的OGTT检测中,rs1884614 T/T基因型与较小的血浆胰岛素曲线下面积存在相关性(P<0.05)。结论:HNF-4α基因的P2启动子区的SNP rs1884614可能影响江苏地区非肥胖型2型糖尿病患者的胰岛素分泌。Objective： To determine whether the single nucleotide polymorphisms（SNPs） of the β-cell type P2 promoter of the HNF-4α gene are associated with type 2 diabetes. Methods： Both rs1884614 and rs2144908 were genotyped in 328 type 2 diabetic patients and 196 non-diabetic controls. The relation of these SNPs to clinical characteristics was also examined. Results： There were no differences in the genotype distribution of the two SNPs between the control and diabetic subjects. However, the rs1884614 T/T geno- type was significantly associated with a smaller area under the plasma insulin curve （ AUC ） during the OGTI＂ in non-obese （ BMI 〈 25 kg/m2 ） patients （P 〈 0.05）. Conclusion： SNP rs1884614 in the P2 promoter re- gion of the HNF-4α gene may influence insulin secretion in non-obese subjects with type 2 diabetes from Jiangsu province.

关 键 词：肝细胞核因子-4α 单核苷酸多态性 2型糖尿病 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]