基于促血管生长的生肌玉红膏有效成分提取工艺的优化  

Optimization of the process on extracting the active principle of the Shengji Yuhong plaster based on improving vascular growth

作　　者：吴玲[1] 吴旭彤[2] 姚昶 孙蕾[2] 张媞 顾铭 

机构地区：[1]南京中医药大学附属医院检验科,江苏南京210029 [2]南京中医药大学附属医院药剂科,江苏南京210029 [3]南京中医药大学附属医院乳腺病科,江苏南京210029

出　　处：《江苏大学学报（医学版）》2016年第2期180-184,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81373646);江苏省中医药管理局康缘科技创新基金资助项目(HZ1009KY);江苏高校优势学科建设工程资助项目(012062003010)

摘　　要：目的:优化基于促进血管生长的生肌玉红膏有效成分的提取工艺。方法:采用人脐静脉血管内皮细胞增殖实验作为药效学指标,比较采用油提法、水提法、醇提法提取出的生肌玉红膏有效成分对促进血管生长的疗效,对筛选出的提取方法的工艺进行药效学比较,确定生肌玉红膏有效成分提取最优工艺流程。结果:优化的生肌玉红膏有效成分提取工艺:取当归、甘草用适量70%乙醇回流提取,提取液回收乙醇至无醇味,备用。另取白芷、紫草二味,以适量70%乙醇作溶剂进行渗漉提取,收集渗漉液,将血竭加入渗漉液中,搅匀,使溶解,再加入当归、甘草提取物,搅匀使溶解,得生肌玉红膏有效成分。结论:采用醇提法得到的生肌玉红膏有效成分促进血管生长的疗效高于传统提取工艺。Objective： To optimize the process on extracting the active principle of the Shengji Yuhong plaster based on the effects of improving vascular growth. Methods： The proliferation of human umbilical vein endothelial cells was taken as pharmacodynamics index to compare the advantage of extracting the ac- tive principle of the Shengji Yuhong plaster in angelica sinensis, liquorice, radix angelicae and lithosper- mum by water extraction, oil extraction and alcohol extraction respectively, followed by investigating the effects of angiogenesis with reflux or diacolation on each composed herbs to determine the optimal process for preparation of the active principle of Shengji Yuhong plaster. Results： The optimal processes for preparation of the active principle of Shengji Yuhong plaster were as follows ： angelica sinensis and liquorice were mixed and extracted by the right amount of 70% ethanol. And radix angelieae and lithospermum were mixed with right amount of 70% ethanol followed by percolation extraction. Then daemonorops draco was mixed homog- enized in the percolation and added with the extractions of angelica sinensis and liquorice finally to complete the whole process. Conclusion： The preparation process of alcohol extraction for the active principle of Shengji Yuhong plaster showed better angiogenic effects and good quality control than those of traditional preparing process.

关 键 词：生肌玉红膏 有效成分 血管生长 醇提法 工艺 优化 

分 类 号：R284.2[医药卫生—中药学]