肉苁蓉多糖水解产物单糖组分的气相色谱法测定  

Determination of monosaccharides composition of Cistanche tubulosa polysaccharides by gas chromatography

作　　者：王鑫彤[1] 王未[2] 吴慧玉 汪松美[4] 刘红阳[4] 李倩[2] 贾清东 张胜洋 赵婷[1] 张敏[1] 仰榴青[1] 

机构地区：[1]江苏大学化学化工学院,江苏镇江212013 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]江苏大学药学院,江苏镇江212013 [4]江苏大学环境与安全工程学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2016年第3期254-257,共4页Journal of Jiangsu University：Medicine Edition

摘　　要：目的：研究气相色谱法测定肉苁蓉多糖中单糖组分的方法。方法：采用单因素实验,考察各因素对肉苁蓉多糖水解的影响;采用气相色谱法测定肉苁蓉多糖中的单糖组成。结果：肉苁蓉多糖水解的最佳条件为硫酸浓度3.0 mol/L,水解时间8 h和水解温度110℃;各单糖精密度RSD为0.92%4.68%,重复性RSD为0.92%1.77%,稳定性RSD为1.61%3.61%,加样回收率为99.3%100.3%,精密度、重复性、稳定性和回收率良好;肉苁蓉多糖主要由甘露糖、葡萄糖和半乳糖组成,摩尔比为2.01∶5.72∶2。结论：此法适用于肉苁蓉多糖中单糖组成分析。Objective ： To explore the optimal methods for analysis of the monosaccharides composition of Cistanche tubulosa polysaccharide. Methods： To investigate modifying factors of the polysaccharide hydroly- sis was determined by single factor method, and the monosaccharide composition analysis of Cistanche tubu- losa polysaccharide by gas chromatography. Results： The optimal hydrolysis conditions were sulfuric acid concentration 3.0 mol/L, hydrolysis time 8 h and temperature 110 ℃ ;the RSD value of precision was be- tween 0.92% and 4.68% ,the RSD value of repeatability was between 0.92% and 1.77% , the RSD value of stability was between 1.61% and 3.61%, the recovery rate were between 99. 3% and 100. 3% ; Cistanche tubulosa polysaccharide was mainly composed of mannose, glucose and galactose in a molar ratio of 2.01 ： 5.72 ： 2. Conclusion： The refined method can be used for monosaccharides composition analy- sis of Cistanche tubulosa polysaccharides.

关 键 词：肉苁蓉 多糖 气相色谱法 单糖组成 

分 类 号：R284.1[医药卫生—中药学]