柿子叶对高血脂大鼠氧自由基及血脂代谢酶的影响  

Influence of Persimmon Leaves on Oxyen Free Radicals and Lipid Metabolism Enzyme in Hyperlipoidemic Model Rats

作　　者：潘朝旺[1] 王伟[2] 谢耀峰[1] 祁学章[2] 江玲兴 

机构地区：[1]鄂州职业大学医学院,湖北鄂州436000 [2]鄂州市中心医院,湖北鄂州436000

出　　处：《中医药导报》2016年第20期42-44,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖北省教育厅科学技术研究项目(B2015502)

摘　　要：目的:观察柿子叶水提液对高血脂大鼠血脂、氧自由基及血脂代谢酶的影响。方法:60只SD大鼠,随机分成6组,正常组,模型组,血脂康组,高、中、低剂量柿子叶组,建立高血脂大鼠模型。药物干预42 d后,测定血清总胆固醇(TC)、甘油三酯(TG)及高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、肝脂酶(HL)、脂蛋白脂酶(LPL)。测定肝脏SOD、GSH-Px、MDA、HL和心脏SOD、GSH-Px、MDA、LPL。结果:柿子叶水提液可明显降低高血脂大鼠模型大鼠血清TG、TC、LDL-C浓度(P<0.05或P<0.01),升高HDL-C浓度(P<0.05或P<0.01)。可明显提高血清、肝脏和心脏SOD、GSH-Px活性(P<0.05或P<0.01),降低血清、肝脏和心脏MDA含量(P<0.05或P<0.01)。可明显升高肝脏HL水平(P<0.05或P<0.01)。对血清和心脏LPL无明显影响。结论:柿子叶具有降血脂作用,其机制与降低体内氧自由基和提高HL水平有关。Objective： To observe the influence of water extract from persimmon leaf on blood lipids, oxyen free radicals and lipid metabolism enzyme in hyperlipoidemic model rats. Methods： The 60 SD rats were randomly divided into 6 groups, including normal group, model group, Xuezhikang group and high, middle and low dose persimmon leaf group. Hypedipoidemic model were established. After 42 days, the serum levels of triglyc- eride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, hepatic lipase （HL）, lipoprotein lipase （LPL）, superoxide dismutase （SOD）, glutathione peroxdase （GSH- Px） and malonaldehyde （MDA） were determined. The SOD, GSH-Px, MDA and HL of liver and the SOD, GSH- Px, MDA and LPL of heart were also determined. Results： The water extract of persimmon leaf can decrease the TG, TC and LDL-C of seru（P〈0.05 or P〈0.01）, and increase the HDL-C obviously （P〈0.05 or P〈0.01）. The water extract of persimmon leaf can increase the SOD and GSH-Px of serum, liver and heart, and decrease the MDA obviously （P〈0.05 or P〈0.01）. The water extract of persimmon leaf can increase the HL of serum and liver obviously （P〈0.05 or P〈0.01）. It showed no obvious effect on LPL of serum and heart. Conclusion： The water extract of persimmon leaf can decrease the level of hyperlipidemia. The mechanism perhaps was related to decrease oxygen free radicals and improve the level of HL.

关 键 词：柿子叶水提液 氧自由基 血脂代谢酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]