基于RNA-Seqs的乌苏里貉agouti基因结构特征预测及分子进化分析  

Gene Structure Prediction and Molecular Evolution Analysis of Raccoon Dog agouti Gene based on RNA-Seqs

作　　者：张浩[1,2] 赵家平[2] 吴琼[2] 杨颖[2] 杜站宇 郭跃跃[2] 邢秀梅[2] 徐超[2] 张林波[1] 

机构地区：[1]吉林农业大学,长春130118 [2]中国农业科学院特产研究所,农业部特种经济动物遗传育种与繁殖重点实验室,吉林省特种经济动物分子生物学省部共建国家重点实验室,长春130112

出　　处：《中国畜牧兽医》2016年第10期2527-2533,共7页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省科技发展计划(20130206029NY);中国农业科学院科技创新工程(ASTIP-ISAPS01);农业部畜禽遗传资源重点实验室开放课题(nzdsys2013-3)

摘　　要：为了进一步分析agouti基因的分子结构特征,利用生物信息学方法对RNA-Seqs试验获得乌苏里貉agouti基因的完整编码区序列的碱基组成及其编码蛋白的结构特征进行了预测和分析,并采用非对组算数平均法(UPGMA)法构建系统发育树。结果表明,获得的乌苏里貉agouti基因cDNA长度为530bp,含396bp开放阅读框(ORF),编码131个氨基酸。预测乌苏里貉agouti蛋白分子质量为14.41ku,等电点(pI)为9.68,为不稳定疏水性蛋白;含有11个磷酸化位点、1个糖基化位点和1个长达24个氨基酸的信号肽;预测发现无规则卷曲为其主要二级结构。系统进化树结果显示乌苏里貉与赤狐、家犬遗传距离最近,这与传统的动物分类学一致。通过对乌苏里貉agouti基因分子结构特征的分析,为揭示其影响毛色多样性的分子遗传学机制提供理论依据。To in-depth analyze the molecular structure characteristics of agouti gene, using bioin- formatic methods for RNA-Seqs test to get the complete coding sequence of raccoon dog agouti gene,and its coding protein structure was forecasted and analyzed. The results showed that the length of raccoon dog agouti gene eDNA was 530 bp which contained 396 bp open reading frame （ORF） ,encoded 131 amino acids. Predicting the weight of the raccoon dog agouti protein molecu- lar was 14.41 ku and isoelectric point （pI） was 9.68,as the unstable hydrophohbic protein;It con- tained 11 phosphorylation sites, 1 glycosylation sites and a long reach 24 amino acid signal pep- tide;Random coil was the main secondary structure. Phylogenetic tree showed that the raccoon dog had a close genetics distance to vuipe and canis familiaris which was consistent with the tradi- tional animal taxonomy. Through the analysis of the molecular structure characteristics of raccoon dog agouti gene, to provide the oretical basis for revealing the in{luence of the colour diversity molecular genetics mechanism.

关 键 词：貉 agouti基因 agouti信号蛋白 生物信息学 

分 类 号：Q78[生物学—分子生物学]