8-oxodG在口腔黏膜白斑中的表达  

Expression of 8-oxod G in Oral Leukoplakia

作　　者：苏秋香[1] 姚波[2] 王旭光[3] 杨丽[4] 张素君[5] 丁晓慧[6] 

机构地区：[1]沈阳医学院基础医学院形态学实验室,辽宁沈阳110034 [2]沈阳市第四人民医院口腔科 [3]沈阳医学院基础医学院病理解剖学教研室 [4]沈阳医学院附属卫生学校口腔学教研室 [5]沈阳医学院基础医学院 [6]沈阳医学院组织与胚胎学教研室

出　　处：《沈阳医学院学报》2016年第5期325-327,331,共4页Journal of Shenyang Medical College

基　　金：辽宁省教育厅科研课题(No.L2014406);沈阳医学院大学生课题(No.20150806)

摘　　要：目的:研究8-氧-7,8-二氢-2'-脱氧鸟嘌呤核苷(8-oxo-7,8-dihydro-2'-deoxyguanosine,8-oxod G)在口腔黏膜白斑中的表达,探讨氧化性DNA损伤在口腔黏膜白斑发生发展中的作用及机制。方法:应用免疫荧光技术检测10例正常口腔黏膜和20例口腔黏膜白斑中8-oxod G和抑癌基因P53蛋白的表达。结果:HE染色可见口腔黏膜白斑中炎性细胞浸润,过角化和上皮异型性增生等组织病理学变化。免疫荧光标记显示8-oxod G在口腔黏膜白斑中生成,并可见抑癌基因P53蛋白的表达。口腔黏膜白斑中8-oxod G和P53蛋白的阳性表达率高于正常口腔黏膜(P<0.01);8-oxod G、P53在正常口腔黏膜、口腔黏膜白斑伴或不伴异型性增生的阳性表达程度具有相关性,与病变程度呈正相关(r=0.773,P<0.01)。结论:氧化性DNA损伤在口腔黏膜白斑的发生过程中起着重要作用,8-oxod G可作为口腔黏膜白斑病变程度的风险性预测标志物。Objective：To study the expression of 8-oxo-7,8-dihydro-2＇-deoxyguanosine （8-oxodG） in oral leukoplakia for clarifying the role of oxidative DNA damage in the development of oral leukoplakia. Methods：Immunofluorescence labeling method was used to examine the expression of 8-oxodG,a marker of oxidative DNA damage,and the expression of tumor suppressor gene, P53 protein in oral epithelium of normal oral mucosa and biopsy specimens of leukoplakia. Results：In specimens of oral leukoplakia, HE staining showed infiltration of inflammatory cells, hyperkeratosis and epithelial dysplasia. Immunofluorescence labeling study demonstrated that the accumulation of 8-oxodG apparently increased in the oral epithelium of patients with leukoplakia,whereas little or no immunoreactivity was observed in normal oral mucosa. Expression of P53 protein was also observed in oral epithelium of patients with oral leukoplakia. The immunoreactivity of 8-oxodG and P53 was stronger in patients with oral leukoplakia than that in normal oral mucosa （P〈0.01） . Moreover,the immunoreactivity increased with the development of disease （r=0.773, P〈0.01） . Conclusions：The oxidative DNA damage contributes to the development of oral leukoplakia. 8-oxodG may be a risk predictive marker for oral leukoplakia.

关 键 词：口腔黏膜白斑 8-氧-7 8-二氢-2'-脱氧鸟嘌呤核苷 氧化性DNA损伤 P53 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]