富马酸氯马斯汀在肺缺血/再灌注损伤中对TLR4表达的影响  

Effects of Clemastine Fumarate on Expression of Toll Like Receptor 4 in Lung Ischemia-Reperfusion

作　　者：刘宁[1] 王薇[1] 张蕊[1] 韩慧蓉[1] LIU Ning WANG Wei ZHANG Rui HAN Hui-rong.(Dept of Anesthesiology ,Weifang Medical University, Weifang 261000, China)

机构地区：[1]潍坊医学院麻醉学系,261000

出　　处：《实用医学杂志》2016年第18期2988-2991,共4页The Journal of Practical Medicine

基　　金：山东省教育厅课题(编号:J13LL02;J15LL53)

摘　　要：目的:探讨肺缺血再灌注损伤时肺组织中Toll样受体4(TLR4)的表达变化及富马酸氯马斯汀对其影响。方法:50只健康清洁级家兔随机分成5组:假手术组(N+S)、缺血1 h再灌注2、4 h组(N+I1/R2、N+I1/R4)、富马酸氯马斯汀干预缺血1 h再灌2、4 h组(F+I1/R2、F+I1/R4)。采用RT-PCR、Western blot和免疫荧光染色法检测肺组织中TLR4表达水平,电镜检测肺组织损伤程度。结果:与假手术组相比,缺血再灌注组TLR4表达量升高(P<0.05),并且再灌4 h组比再灌2 h组TLR4表达量增加更为显著(P<0.05);使用富马酸氯马斯汀干预后TLR4表达被明显抑制(P<0.05)。结论:肺缺血再灌注损伤可促进肺组织TLR4表达,并产生严重肺组织损伤,且与再灌注时间成正相关;富马酸氯马斯汀可以有效抑制肺组织TLR4的表达,显著减轻肺组织损伤。Objective To explore the expression change of Toll Like Receptor 4 （TLR4） in lung ischemia-reperfusion injury in rabbits and the influence of clemastine fumarate on it. Methods Fifty rabbits were divided into five groups randomly （n=10）： Sham （N＋S）, reperfusion for 2 hours （N＋I1/R2）, reperfusion for 4 hours （N＋I1/R4）, clemastine fumarate ＋ reperfusion for 2 hours （F＋I1/R2） and clemastine fumarate＋ reperfusion for 4 hours （F＋I1/R4）. The ischemia time in each group was 1 hour and normal saline was given respectively in groups of N＋S, N＋I/R2 and N＋I1/R4. Western blotting, RT-PCR and imnmnofluorescence were used to detect the expression of TLR4 in lung tissue, and the changes of uhrastrncture in ischemia-reperfusion lung tissue were observed by electron microscope. Result The expression of TLR4 was elevated obviously in ischemia-reperfusion lung tissue （P〈0.05）, and there was positive correlation between the increased TLR4 level and reperfusion time （P〈0.05）, the swelled and thick-ridge mitochondria were observed in type II alveolar epithelial cells after LIRI （P〈0.05） ; but clemastine fumarate inhibited the expression of TLR4 in lung tissue significantly caused by LIRI （P〈0.05）. And the mitochondria injury was reduced in the groups of clemastine fumarate. Conclusion TLR4 expression is elevated in lung tissue after LIRI; clemastine fumarate inhibits the expression of TLR4 caused by LIRI and protects the lung tissue from LIRI in rabbits.

关 键 词：TLR4 富马酸氯马斯汀 肺缺血再灌注损伤 肺保护 

分 类 号：R563[医药卫生—呼吸系统]