雌二醇对临床表皮葡萄球菌生长和生物膜形成的作用  

Estradiol stimulates the growth and biofilm formation of clinical Staphylococcus epidermidis

作　　者：王曦[1] 周永春 黄云超[3] 邹天宁[1] 吕志勇[4] 陈颖[3] 刘馨[5] 段万石 

机构地区：[1]昆明医科大学第三附属医院/云南省肿瘤医院乳腺外一科,650118 [2]云南省肺癌研究重点实验室 [3]昆明医科大学第三附属医院胸外一科 [4]昆明医科大学第三附属医院麻醉手术科 [5]昆明医科大学第三附属医院肿瘤研究所

出　　处：《中华医学杂志》2016年第38期3083-3089,共7页National Medical Journal of China

摘　　要：目的探讨雌二醇对乳房假体表面表皮葡萄球菌生长、生物膜形成的影响。方法以乳房硅胶片为载体，选择表皮葡萄球菌标准株（ATCC）和临床株（SE），在不同雌二醇浓度下培养4、6、12、24、48和72 h。根据吸光度（A）值绘制出生长曲线；用结晶紫染色法检测生物膜形成能力；用激光聚焦显微镜（CLSM）和扫描电子显微镜（SEM）观察生物膜结构。结果4株细菌在加入雌二醇后生长都较空白组提前1 h进入对数生长期（雌二醇组在第3小时进入，空白组在第4小时进入）。ATCC12228和SE40在高浓度雌二醇作用下仍无生物膜形成（A值均〈0.12）。ATCC35984和SE101在雌二醇作用下，初始黏附能力明显加强，0 pmol/L组（0.081±0.015和0.082±0.011），50 pmol/L组（0.087±0.013和0.088±0.010），125 pmol/L组（0.175±0.052和0.091±0.012），250 pmol/L组（0.153±0.036和0.090±0.006），500 pmol/L组（0.157±0.050和0.082±0.032）；同时细菌生物膜厚度也随雌二醇浓度而增加，培养24 h时的A值分别为0 pmol/L组（1.609±0.171和0.247±0.017），50 pmol/L组（1.391±0.087和0.256±0.015），125 pmol/L组（2.051±0.070和0.268±0.064），250 pmol/L组（1.973±0.073和0.265±0.019），500 pmol/L组（1.904±0.060和0.245±0.019），在雌二醇浓度为125 pmol/L时生物膜达到最厚。CLSM和SEM观察到高浓度的雌二醇能促使生物膜提前进入成熟阶段，125 pmol/L雌二醇组的生物膜较其他浓度组形成的生物膜更致密、更厚。ObjectiveTo investigate the effect of steroid hormones on the growth and biofilm formation of S. epidermidis.MethodsIn all experiments, two S. epidermidis standard strains （ATCC12228 and ATCC35984） and two S. epidermidis clinical strains （SE101 and SE40） were incubated on the surface of silica gel in estradiol concentrations of 0, 50, 125, 250 and 500 pmol/L at 4, 6, 12, 24, 48 and 72 hours. Growth curve were drawn by means of the OD value. Formed biofilms were assessed by crystal violet staining methods. In order to observe the processes and structures of biofilm, the confocal laser scanning microscopy （CLSM） and scanning electron microscope （SEM） were used.ResultsAll of the four strains which were incubated with the concentrations of 125 pmol/L of estradiol had entered the logarithmic growth stage ahead of the blank control （3 h and 4 h）. Crystal violet staining indicated that there was no biofilm for ATCC12228. Improved primary attachment abilities were found for ATCC35984 and SE101 in the presence of estradiol, and the OD values were 0 pmol/L （0.081±0.015 and 0.082±0.011）, 50 pmol /L （0.087±0.013 and 0.088±0.010）, 125 pmol/L （0.175±0.052 and 0.091±0.012）, 250 pmol/L （0.153±0.036 and 0.090±0.006）, 500 pmol/L （0.157±0.050 and 0.082±0.032）, respectively. The thickness of biofilm reached the peak in 125 pmol/L estradiol at 24 h. CLSM and SEM found estradiol promoted the biofilm maturity and the biofilm of 125 pmol/L group was denser and thicker than that of the other concentration groups.ConclusionsOur in vitro experiments indicate that estradiol could promote the growth of S. epidermidis which exit on the surface of silica gel, and improve the ability of biofilm formation of S. epidermidis.

关 键 词：雌二醇 乳房假体 表皮葡萄球菌 细菌生长 生物膜 

分 类 号：R655.8[医药卫生—外科学]