ELISA法在诊断梅毒螺旋体感染中的临床检验价值  

Chinical laboratory value of ELISA in diagnosis of Treponema pallidum infection

作　　者：孟保福 宋红林[1] 李翠芬[1] 

机构地区：[1]安阳市人民医院检验科,河南安阳455000

出　　处：《中华医院感染学杂志》2016年第20期4611-4613,共3页Chinese Journal of Nosocomiology

基　　金：河南省科技厅科技攻关基金资助项目(112102310693)

摘　　要：目的探讨酶联免疫吸附试验(ELISA)在梅毒螺旋体感染诊断中的检验价值,为临床中诊断梅毒螺旋体感染提供可靠的参考依据。方法选取医院2013年1月-2015年12月90例疑似梅毒螺旋体感染患者为研究对象,患者进行ELISA检查和甲基胺红不加热血清试验(TRUST)检查,以临床最终检查结果作为金标准,分析ELISA临床应用价值。结果 TRUST检查诊断准确率为83.3%,ELISA检查诊断准确率为95.6%,ELISA检查诊断准确率明显高于TRUST检查,差异有统计学意义(P<0.05);TRUST检查梅毒螺旋体感染灵敏度为83.3%、特异度为83.3%,低于ELISA检查梅毒螺旋体感染灵敏度为95.8%、特异度为94.4%,数据差异有统计学意义(P<0.05)。结论临床中对于梅毒螺旋体感染患者应用ELISA诊断具有较好的临床效果,具有较高的诊断价值。OBJECTIVE To investigate the enzyme-linked immunodeficient assay （ELISA） test value method in the diagnosis of Treponema pallidum infection, so as to provide a reliable reference for the clinical diagnosis of T. pallidumi nfection. METHODS A total of 90 cases of suspected T. pallidum infection patients in our hospital from Jan. 2013 to Dec. 2015 were selected as research subjects, who were under ELISA examination and methyl red unheated serum test （TRUST） to get clinical findings as the gold final standard and the clinical value of ELISA was analyzed. RESULTS TRUST diagnosis accuracy rate was 83. 3%, and ELISA diagnosis accuracy rate was 95.6%, so ELISA diagnosis accuracy rate was significantly higher than TRUST（P〈0.05）. The sensitivity of TRUST check to T. pallidum infection was 83.3 %, and specificity was 83.3 %, which were lower than ELISA sensitivity 95.8% and specificity 94.4%, and the difference was significant（P〈0. 05）. CONCLUSION The clinical application of ELISA to diagnosis T. pallidum infection has a good clinical effect, with higher diagnostic value.

关 键 词：梅毒螺旋体 感染 酶联免疫吸附试验 诊断价值 

分 类 号：R514[医药卫生—内科学]