南京地区腹泻婴幼儿新型诺如病毒GII.17变异株的检出及基因分析  

Detection and genetic analysis of new GII.17 norovirus variant from infantile diarrhea in Nanjing

作　　者：蒋翠莲[1] 叶莉莉[1] 许烨[1] 顾平清[1] 张洪英[2] 靳淼 艾静[4] 付建光[4] 

机构地区：[1]南京市妇幼保健院检验科,江苏南京210004 [2]南京市疾病预防控制中心急传科,江苏南京210003 [3]国家疾病预防控制中心病毒病所,北京100052 [4]江苏省疾病预防控制中心急传所,江苏南京210009

出　　处：《中华医院感染学杂志》2016年第20期4717-4720,共4页Chinese Journal of Nosocomiology

基　　金：十二五科教兴卫工程基金资助项目(ZX201109);江苏医学重点人才基金资助项目(RC2011084)

摘　　要：目的了解南京地区腹泻婴幼儿新型GII.17诺如病毒变异株的检出及其基因特征,为疾病预防控制及疫苗研制提供切实有力的科学依据。方法收集2015年1-6月南京市妇幼保健院疑似病毒性腹泻粪便标本178份,采用荧光定量PCR方法对标本进行诺如病毒检测并对阳性标本进行基因分析。结果 178份粪便标本中,检出GII型诺如病毒27份,阳性率为15.2%,未检测出GI型诺如病毒,对该地区诺如病毒阳性标本进行衣壳蛋白区测序,发现27份GII标本中14株为新型GII.17变异株,8株为GII.3型,5株为GII.4-Sydney型;对其中5株GII.17变异株进行完整的衣壳蛋白VP1测序,发现其VP1区部分氨基酸已经出现了突变,而有些氨基酸位点的突变是与病毒抗原性及人群易感性密切相关。结论新型GII.17诺如病毒变异株在南京地区患病毒性腹泻的婴幼儿中检出,且检出率高于其他诺如病毒株,表明其可能已成为本地区新的诺如病毒流行变异株,考虑到其已在国内引起暴发流行,提示该毒株将会是本地区在今后重点预防控制监测的诺如病毒株。OBJECTIVE To investigate new GII. 17 norovirus （NOV） variant infection status and indentify its genet- ic characteristics in Nanjing, so as to provide references for disease control and prevention and vaccine develop- ment. METHODS A total of 178 stool specimens were collected from the outpatient children with acute diarrhea ad- mitted to the Nanjing Maternity and Child Health Care Hospital in Nanjing from Jan. to Jun. 2015. NoV gene- groups were detected by real-time PCR （RT-PCR）, and genotypes were determined by sequence analysis. RESULTS Among 178 stool specimens, 27 （15.2%） cases were positive for NoV GII, and GI was not found. Genotype study was conducted by analyzing the nucleotide sequence of capsid gene. Based on capsid region, 14 strains were new GII. 17 variants, 8 strains were GII. 3 and 5 strains were GII. 4-Sydney, respectively. Further- more, we amplified VPlgene of five variants and compared with epidemic strains in amino acid sequence. All of them had mutations in some key sites in amino acid sequence, which were closely related to form neutralizing epitopes and population susceptibility. CONCLUSION The new GII. 17 norovirus variant had been detected in young children with infantile viral diarrhea in Nanjing, which detection rate was higher than other norovirus variants, and it might have become the dominant strain of NoV. Considering that it had already triggered epidemic in China, the monitoring of this novel GII. 17 variant will be a crucial part of NoV surveillance in Nanjing.

关 键 词：诺如病毒 变异株 GII.17型 基因分析 

分 类 号：R725.1[医药卫生—儿科]