美替拉酮对放线菌Streptomyces sp.LXF-80代谢途径的影响  

Study on the treatment of Streptomyces sp.LXF-80 with P450 inhibitor metyrapone

作　　者：王皓天[1] 刘晓芳[1] 朱天骄[1] 李德海[1] 顾谦群[1] 车茜[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室医药学院,山东青岛266003

出　　处：《中国海洋药物》2016年第5期29-34,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(21402180);中国海洋大学青年教师科研专项基金项目(201413013)资助

摘　　要：目的渤海海泥来源放线菌Streptomyces sp.LXF-80可以生产罕见的包含α-吡喃酮的三环骈合聚酮化合物肠道菌素(Enterocin)。美替拉酮是1种细胞色素P450氧化酶的抑制剂,本实验试图利用美替拉酮影响肠道菌素的生合成过程,表达获得新的聚酮类化合物。方法向LXF-80的发酵培养基中添加酶抑制剂,结合HPLC分析确定最优条件,并进行大规模发酵和目标产物的分离。结果从添加美替拉酮的LXF-80发酵提取物中共分离获得3个化合物,包括Enterocin(1)、5-deoxyenterocin(2)以及3-epi-5-deoxyenterocin(3)。结论实验结果证明美替拉酮的添加,影响了菌株聚酮类化合物的生合成过程,得到2个肠道菌素的形成过程中的中间体化合物2和3。Objective Our recent investigation of marine-derived strain Streptomycessp.LXF-80 resulted in the isolation of complicated tricyclicpolyketide enterocin.Metyrapone was a kind of cytochrome P450 enzyme inhibitor.In this paper,the novel polyketide compounds were detected by affecting the biosynthesis pathways of the strain,based on the treatment with metyrapone.Methods Various enzyme inhibitors were fed into the medium of Streptomycessp.LXF-80,the optimal conditions were determined according to the HPLC analysis,and the aim compounds were separated.Results Enterocin（1）,its two derivatives 5-deoxyenterocin（2）and 3-epi-5-deoxyenterocin（3）were separated.Conclusion Metyrapone could actually affect the metabolic pathways of Streptomyces sp.LXF-80.

关 键 词：肠道菌素 美替拉酮 酶抑制剂 

分 类 号：R931[医药卫生—生药学]