丁酰化甲壳素的制备及其生物相容性研究  

The study on preparation and biocompatibility of butyrylchitin

作　　者：石杰[1] 李哲[1] 陈全[1] 蒋志雯[1] 彭燕飞[1] 韩宝芹[1] 刘万顺[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋药物》2016年第5期74-80,共7页Chinese Journal of Marine Drugs

基　　金：国家高技术研究发展计划课题(2014AA093605);山东省自主创新及成果转化专项(2014ZZCX06203)资助

摘　　要：目的制备丁酰化甲壳素(Butyrylchitin,BC)并评价其生物相容性。方法以甲壳素(Chitin,CT)为材料,正丁酸酐为酰化剂,高氯酸为催化剂制备BC。通过红光光谱、元素分析对BC进行表征,以小鼠成纤维细胞L929和Wistar大鼠为模型评价细胞毒性,组织相容性和降解性。结果红光光谱、元素分析结果表明,甲壳素的羟基氢原子被取代,发生了丁酰化反应,取代度为1.96。细胞相容性评价结果显示,BC对小鼠成纤维细胞L929细胞生长没有影响,与对照组相比无显著性差异(P>0.05)。体内降解性和组织相容性评价结果显示,BC膜片植入大鼠皮下和肌肉,降解缓慢,降解初期有一定的炎症反应,但总体表现出良好的组织相容性。结论成功制备了BC,其具有良好的生物相容性。Objective To synthesize butyrylchitin（BC）and evaluate biocompatibility of BC.Methods BC was synthesized from chitin and butyric anhydride with perchloric acid as catalyst.BC was characterized by FTIR and elemental analysis.L929（Mouse fibroblast cells）and Wistar rat models were used to evaluate the cytotoxicity,biocompatibility and degradation.Results The results of FTIR and elemental analysis investigation showed that BC with degree of substitution closed to 2was obtained.L929 were cultured on BC membrane in vitro to evaluate effect of cells growth.The result indicated that there was no significant difference,compared with control group（P〈0.05）.The BC membrane degraded slowly in vivo,and had some inflammatory reaction at the initial stage.Conclusion The BC material was synthesized successfully and showed a good biocompatibility on the whole.

关 键 词：丁酰化甲壳素 细胞毒性 生物相容性 

分 类 号：R931[医药卫生—生药学]