孕20周前孕妇血浆中色素上皮衍生因子表达变化与子痫前期关联性研究  

Study on the correlation between change of maternal plasma pigment epithelium derived factor expression and preeclampsia before the 20th gestational week

作　　者：江敏[1] 杨红玲[1] 龙燕[1] 赵学芹[1] 

机构地区：[1]广州医科大学附属广州市妇女儿童医疗中心检验部,广东广州510623

出　　处：《中国妇幼保健》2016年第20期4119-4122,共4页Maternal and Child Health Care of China

基　　金：广东省科技厅项目(2016A020218004);广东省自然基金项目(2014A030313004);广州市科技攻关项目(201300000135)

摘　　要：目的探讨孕20周前孕妇血浆中色素上皮衍生因子蛋白表达水平与子痫前期的关系。方法采用前瞻性巣式病例对照研究方法。选取在广州市妇女儿童医疗中心产科20周前的单胎子痫前期孕妇68例,分为子痫前期轻度组34例,子痫前期重度组34例;以同期分娩的健康妊娠孕妇68例作为对照组。采用ELISA法分析孕20周前色素上皮衍生因子(PEDF)的表达水平,同时结合临床风险因素分析PEDF对子痫前期的临床预测价值。结果子痫前期轻度组、子痫前期重度组、对照组血浆中PEDF的表达量分别为:(12.02±3.34)μg/ml、(12.42±4.34)μg/ml,(10.21±2.88)μg/ml,子痫前期轻度组及重度组PEDF的表达量显著高于对照组,差异有统计学意义(P<0.05);子痫前期轻度组与重度组PEDF表达量比较,差异无统计学意义(P>0.05)。回归分析:子痫前期组中孕妇的PEDF、孕前BMI、贫血可作为预测子痫前期的综合指标。结论子痫前期孕妇孕20周前血浆中PEDF蛋白高表达,提示PEDF可能与子痫前期的发生相关;PEDF结合孕前BMI、贫血等临床风险因素对子痫前期的预测诊断有更高的价值。Objective To explore the relationship between expression level of maternal plasma pigment epithelium derived factor（ PEDF） and preeclampsia before the 20 th gestational week. Methods A prospective nested case-control study was conducted to select 68 women of single pregnancy with preeclampsia before the 20 th gestational week from Department of Obstetrics in the hospital,then they were divided into mild preeclampsia group（ 34 patients） and severe preeclampsia group（ 34 patients）; 68 healthy pregnant women giving birth to their babies during the same period were selected as control group. The expression levels of PEDF before the 20 th gestational week were detected by ELISA. Combining with clinical risk factors,the clinical predictive value of PEDF for preeclampsia was analyzed. Results The expression levels of plasma PEDF in mild preeclampsia group,severe preeclampsia group,and control group were（ 12. 02± 3. 34） μg / ml,（ 12. 42±4. 34） μg/ml,and（ 10. 21±2. 88） μg/ml,respectively,the expression levels of PEDF in mild preeclampsia group and severe preeclampsia group were statistically significantly higher than that in control group（ P〈0. 05）,there was no statistically significant difference between mild preeclampsia group and severe preeclampsia group（ P〉 0. 05）. Regression analysis showed that PEDF,progestational body mass index（ BMI）,and anemia could be used as comprehensive indexs of prediction for preeclampsia. Conclusion High expression of plasma PEDF in pregnant women with preeclampsia before the 20 th gestational week indicates that PEDF may be involved in occurrece of preeclampsia; PEDF combined with clinical risk factors（ such as BMI,anemia） has high value in prediction of preeclampsia.

关 键 词：子痫前期 色素上皮衍生因子 妊娠 

分 类 号：R714.245[医药卫生—妇产科学]