枣仁安神方配伍变化对五味子醇甲及五味子甲素药动学的影响  

Influence of composition changes in Jujube Anshen Prescription on pharmacokinetics of schizandrin and deoxyschizandrin

作　　者：张丽[1] 李珊[1] 赵阿静 张笑笑[1] 高蓉[1] 王晓峰[1] 房敏峰[1] 

机构地区：[1]西部资源生物与现代生物技术教育部重点实验室,西北大学生命科学学院,陕西西安710069

出　　处：《中国医院药学杂志》2016年第19期1636-1640,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:J1210063);教育部创新团队发展计划项目(编号:IRT_15R55);陕西省重点科技创新团队计划(编号:2013KCT-24)

摘　　要：目的：考察枣仁安神方配伍变化对五味子醇甲和五味子甲素药动学影响。方法：SD大鼠分为五味子组、五味子-丹参组、五味子-酸枣仁组及枣仁安神方组,灌胃给药,采用高效液相色谱法（HPLC）对0-24h内大鼠血浆中五味子醇甲和五味子甲素测定,DAS 3.0软件计算药动学参数。结果：与五味子组相比,五味子-丹参组,五味子醇甲CL/F明显增加,五味子甲素V1/F显著减小;五味子-酸枣仁组,五味子醇甲AUC（0-t）显著降低,五味子甲素t1/2Ka值明显减小,而枣仁安神方组五味子醇甲和五味子甲素t1/2Ka值均明显增加,五味子醇甲AUC（0-t）、AUC（0-∞）显著增加,CL/F、V1/F显著降低。结论：枣仁安神方的不同配伍对五味子醇甲和五味子甲素的药动学存在不同影响,与五味子组相比,复方显著减缓五味子醇甲和五味子甲素的消除,延长了作用时间。OBJECTIVE To investigate influences of composition changes of Jujube Anshen prescription on pharmacokinetics of schizandrin and deoxyschizandrin.METHODS SD rats were divided into four groups including Fructus Schisandrae chinensis group,Fructus Schisandrae chinensis-Radix Salvia Miltiorrhizae group,Fructus Schisandrae chinensis-Semen Ziziphus spinosa group and prescription group,which were gavaged to rats.Blood samples were collected at 24 h from rats in sequence.HPLC was used to determined concentrations of schizandrin and deoxyschizandrin in plasma 24 h after oral administration.DAS 3.0software was used to calculate pharmacokientic parameters.RESULTS Compared with Fructus Schisandrae chinensis,prescription group had significantly increased area under curve（AUC（0-t）and AUC（0-∞））of schizandrin,clearance speed（CL/F）and distribution volume（V1/F）were reduced,values of absorption half time（t1/2Ka）of schizandrin and deoxyschizandrin were remarkably increased,clearance speed（CL/F）of schizandrin was apparently increased and distribution volume（V1/F）of deoxyschizandrin was reduced in Fructus Schisandrae chinensis-Radix Salvia Miltiorrhizae group and in Fructus Schisandrae chinensis-Semen Ziziphus spinosa group,area under curve（AUC（0-t））of schizandrin and absorption half time（t1/2Ka）of deoxyschizandrin were dramatically decreased.No obvious difference was observed in other parameters.CONCLUSION Different compositions of Jujube Anshen prescription have different influences on pharmacokinetics of schizandrin and deoxyschizandrin.Compared with Fructus Schisandrae chinensis,prescription group has effectively slowed down elimination and prolonged functioning time of schizandrin and deoxyschizandrin in vivo,subsequently.

关 键 词：枣仁安神方 五味子醇甲 五味子甲素 药动学 配伍 

分 类 号：R285.5[医药卫生—中药学]