三叶青藤正丁醇部位对DBA/1小鼠胶原性关节炎的作用及机制  

Effects and mechanism of n-butanol extract fraction of Illigera rhodantha Hance.on collagen induced arthritis in DBA/1 mice

作　　者：黄博[1] 墨虹 蔡小玲[2] 邓俊刚[2] 谭亲友[2] 李江[2] 

机构地区：[1]桂林医学院附属医院风湿免疫科,广西桂林541001 [2]桂林医学院附属医院药学部,广西桂林541001

出　　处：《中国医院药学杂志》2016年第19期1653-1657,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然基金(项目批准号:81460625);广西自然基金(项目编号:2014GXNSFAA118168);广西高等学校重点资助科研项目(合同编号:201202ZD066)

摘　　要：目的:观察三叶青藤正丁醇部位(nbuIR)对胶原性关节炎小鼠关节病变及血清炎症因子的抑制作用。方法:用DBA/1小鼠建立胶原性关节炎(CIA)动物模型,将造模成功小鼠随机分为模型对照组、甲氨蝶呤组、nbuIR低、中、高剂量组,另设正常对照组。灌胃给药,给药期间每周进行一次关节炎指数评分。干预结束后,ELISA法检测各组小鼠血清IL-23和IL-17水平,H-E染色观察各组小鼠踝关节组织病理学改变。结果:与模型对照组比较,nbuIR高剂量组血清IL-23和IL-17水平明显降低(P<0.01),滑膜细胞增生与炎症细胞浸润程度明显减轻。结论:nbuIR能改善CIA小鼠局部关节肿胀,下调血清IL-23和IL-17水平,减少滑膜炎症程度,其机制可能与调节IL-23/IL-17轴有关。OBJECTIVE To observe inhibiting effects of n-butanol extract fraction in Illigera rhodantha Hance.（nbuIR）on pathological changes of ankle joint and serum inflammatory factors in mice with collagen-induced arthritis（CIA）.METHODSCIA model of DBA/1 mice were established and randomly divided into model control group,MTX group,nbuIR low,medium and high dose groups.At the same time,normal control group was also assigned.Drugs were given to mice intragastrically,and arthritis index score was evaluated once a week.After intervention,serum interleukin-23（IL-23）and interleukin-17（IL-17）levels were detected by ELISA,histopathological changes of ankle joint were observed by hematoxylin eosin（H-E）staining in each group.RESULTS Compared with CIA model control group,IL-23 and IL-17 levels of nbuIR high dose group decreased significantly（P〈0.01）,proliferation of synovial cells and infiltration of inflammatory cells were alleviated obviously.CONCLUSION NbuIR can improve local joint swelling of CIA mice,lower serum IL-23 and IL-17 levels,reduce degree of synovitis,which may be correlated with potential mechanism to regulate IL-23/IL-17 axis.

关 键 词：三叶青藤 正丁醇部位 胶原性关节炎 白细胞介素-17 白细胞介素-23 

分 类 号：R285.5[医药卫生—中药学]