重症监护室多重耐药鲍曼不动杆菌整合子与耐药相关性研究  

Research on correlation between integron and antibiotic resistance of multi-drug resistant Acinetobacter baumanii in ICU

作　　者：宋志方[1] 江乐[1] 张亦婷[1] 俞国峰[1] 

机构地区：[1]绍兴市人民医院,浙江绍兴312000

出　　处：《中国医院药学杂志》2016年第19期1693-1696,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:了解鲍曼不动杆菌整合子分布情况,探讨发现的整合子与其耐药性的关系。方法:分离2014—2015年重症监护室(ICU)多重耐药鲍曼不动杆菌,用VITEK2全自动微生物鉴定系统进行菌种鉴定及药敏检测,聚合酶链反应(PCR)检测整合子可变区。结果:鲍曼不动杆菌对ICU常用药物除头孢哌酮舒巴坦外耐药率均超过50%,整合子结构的检出率为82.81%,可变区包含arr-3+aacA4和aacA4+catB8+aadA1,属于两种Ⅰ类整合子。结论:ICU的多重耐药鲍曼不动杆菌广泛存在Ⅰ类整合子结构,介导对氨基糖苷类、氯霉素和利福平耐药,对ICU多重耐药鲍曼不动杆菌的耐药基因有待进一步检测。OBJECTIVE To investigate distribution of integron of Acinetobacter baumanii（Ab）,analyze correlation between integron and drug resistance.METHODS Multi-drug resistant Acinetobacter baumanii was isolated from ICU from2014 to2015.VITEK2 full automatic bio-identification system was used to detect bacterial species and drug sensitivity.Variable regions of integron were detected by PCR.RESULTS Drug resistance rates of Acinetobacter baumanii were higher than50% except cefoperazone/sulbactam in commonly used antibiotics in ICU.Positive rate of integron was 82.81%,two types of class I integron were detected in variable region,which were arr-3＋aacA4 and aacA4＋catB8＋aadA1.CONCLUSION Class I integron widely exists in multi-drug resistant Acinetobacter baumanii in ICU,and mediates resistance to aminoglycosides,chloramphenicol and rifampin.Further researches are need to investigate gene mutation which results in high drug resistance in ICU.

关 键 词：鲍曼不动杆菌 I类整合子 耐药基因盒 

分 类 号：R969[医药卫生—药理学]