金盏菊不同提取液体外抑菌作用初步研究  

Study on the bacteriostatic effects of Jinzhanju extract in vitro

作　　者：郑佳[1] 卢先明[2] 邓晶晶[2] 

机构地区：[1]乐山职业技术学院,四川乐山614000 [2]成都中医药大学,四川成都611137

出　　处：《中药与临床》2016年第3期45-46,共2页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:考察金盏菊水提物和醇提物的体外抑菌作用。方法:采用2倍递增稀释浓度法检测金盏菊对金色葡萄球菌、表皮葡萄球菌、大肠杆菌、绿脓杆菌、沙门氏菌5种菌最低抑菌浓度(MIC)。结果:金盏菊醇提物在10倍稀释浓度时对大肠杆菌、金色葡萄球菌、表皮葡萄球菌有抑菌作用,最低抑菌浓度(MIC)为0.1 g.mL^(-1);金盏菊水提物在10倍稀释浓度时对大肠杆菌有抑菌作用,最低抑菌浓度(MIC)为0.1 g.mL^(-1),其余稀释倍数均无抑菌作用。结论:金盏菊提取液对这5种细菌的抑制效果较差,可能与金盏菊药用部位、提取溶剂及生长环境有关。Objective： To study the bacteriostatic effects of water extract and alcohol extract of Jinzhanju in vitro. Method： Minimal inhibitory concentrations of Jinzhanju extract were determined by double-fold dilution method on staphylococcus aureus, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa and salr, anella typhimurium. Result： The MIC of alcohol extract were 0.1 g. mL-1 for E. coli, S. Aureus and S.epidermidis at 10 times diluted concentration. The MIC of water extract was 0.1 g. mL-1 for E. coli at 10 times diluted concentration.Conclusion： Jinzhanju extract shows poor bacteriostatic effects on the tested bacterium which may be related to the medicinal part, extraction solvent and growth environment.

关 键 词：金盏菊 体外抑菌作用 醇提取液 水提取液 

分 类 号：R285.5[医药卫生—中药学]