PRRSV体外感染PAMs对IFN-γ mRNA转录水平的影响  

The Effects of PRRSV Infected PAMs on Transcription Levels of IFN-γ in Vitro

作　　者：闫晓霞[1] 刘欢欢[1] 曾梦颖 高洪[1] 严玉霖[1] 

机构地区：[1]云南农业大学动物科学技术学院,昆明650201

出　　处：《中国兽药杂志》2016年第10期1-5,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金项目(31160496;31360599);云南农业大学2015年研究生科技创新项目(2015ykc4)

摘　　要：为探讨猪繁殖与呼吸综合征病毒（PRRSV）体外感染猪肺泡巨噬细胞（PAMs）对IFN-γmRNA转录水平的影响,选用PRRSV血清抗体和抗原均为阴性的4周龄健康仔猪3头,无菌分离PAMs,随机将其分为对照组、PRRSV感染组（PRRSV组）、PRRSV＋PHA组和RRRSV＋Dex组,分别在培养6、12、24、48和60 h收集PAMs。运用荧光定量PCR检测IFN-γ和PRRSV mRNA转录情况。结果显示,PRRSV mRNA转录量在感染PAMs后6~24 h逐渐升高,在48 h有所下调,在60 h再次升高,IFN-γmRNA转录量在6~48 h逐渐升高,24、48 h显著高于对照组,而到60 h又恢复到对照组水平;与同时间点的PRRSV组相比,PRRSV＋PHA组中PRRSV mRNA转录量有所下调,IFN-γmRNA转录量有所上调;而RRRSV＋Dex组PRRSV mRNA转录量有所上调,IFN-γmRNA转录量所下调。结果表明,IFN-γ在一定程度上可以抑制PRRSV在PAMs中的增殖,IFN-γ的抑制可能是PRRSV导致细胞损伤的机制之一。To explore the effects of porcine reproductive and respiratory syndrome virus （PRRSV） infected pulmonary alveolar maerophage （PAMs） on transcription levels of IFN - γ in vitro, three four weeks old piglet free of PRRSV was used in this experiment. The PAMs were isolated aseptically and divided into control group, PRRSV infection group （ PRRSV group） , PRRSV ＋ PHA group, PRRSV ＋ Dex group and cultured in vitro. The PAMs were collected at 6, 12, 24, 48 and 60 hours post infection. The mRNA transcription of IFN - γ and PRRSV were analyzed by Real - Time PCR. The results shows that the mRNA levels of PRRSV in PRRSV group were gradually increased at 6 - 24 h and were decreased at 48 h ; the transcription of IFN - -y were gradually increased at 6 -48 and significantly higher at 24 and 48 h than control group. PRRSV ＋ PHA group the mRNA levels of PRRSV were slightly lower and the IFN - γ is higher than PRRSV group; RRRSV ＋ Dex group the mRNA transcription of PRRSV were higher but the IFN - γ is lower. The results indicated IFN - γ proliferation of PRRSV in PAMs, the inhibition of IFN - γ is probably one of the mechanisms that cellular damage Key words： PRRSV ; PAMs; IFN - γ can inhibit the PRRSV cause

关 键 词：猪繁殖与呼吸综合征病毒 肺泡巨噬细胞 干扰素-Γ 

分 类 号：S852.65[农业科学—基础兽医学]