半夏泻心汤含药血清对胃癌腹膜转移细胞系GC9811-P增殖及侵袭转移的影响  

Effects of Banxia Xiexin Decoction Containing Serum on Proliferation,Invasion and Metastasis of Gastric Cancer Peritoneal Metastasis Cell Line GC9811-P

作　　者：刘喜平[1] 李沛清[1] 明海霞[1] 张炜[2] 王强[1] 陈彦文[1] 杨柏林[1] 

机构地区：[1]甘肃中医药大学基础医学院,兰州730000 [2]兰州大学第一医院普外科,兰州730000

出　　处：《中国中西医结合杂志》2016年第10期1224-1228,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81260525);甘肃省高校基本科研业务费专项基金资助项目(No.BH-2001-068)

摘　　要：目的观察半夏泻心汤含药血清对体外人胃癌腹膜高转移潜能细胞株GC9811-P增殖及侵袭转移的影响。方法制备半夏泻心汤含药血清,在E-Plate 96及CIM Plate16检测板孔中接种GC9811-P细胞,并加入0、25、50、100μL/m L的半夏泻心汤含药血清,同时对GC9811-P细胞进行Diff-Quik染色,采用实时无标记细胞分析技术(real time cellular analysis,RTCA)结合Diff-Quik染色法,观察半夏泻心汤对GC9811-P增殖细胞指数(cell index,CI)及侵袭转移的抑制作用。结果半夏泻心汤含药血清可明显抑制GC9811-P细胞增殖,70 h后CI接近于0,Diff-Quik染色细胞大多死亡。细胞迁移曲线表明,25、50、100μL/m L的半夏泻心汤含药血清能明显抑制GC9811-P细胞迁移能力,细胞迁移数目均较0μL/m L半夏泻心汤含药血清明显减少,且呈浓度依赖关系(P<0.05)。结论半夏泻心汤含药血清可抑制体外人胃癌腹膜高转移潜能细胞株GC9811-P的增殖及侵袭转移,可能与其具有阻断胃癌腹膜转移的作用有关。Objective To observe the effects of Banxia Xiexin Decoction（ BXD） containing serum on proliferation,invasion and metastasis ofin vitro human gastric cancer peritoneum cell strain GC9811-P（ which has high metastatic potential）. Methods BXD containing serum was prepared. GC9811-P cells were inoculated in the EPlate 96 and CIM Plate 16,and then 0,25,50,100 μL / m L BXD containing serums were added respectively.Meanwhile,GC9811-P cells were stained by Diff-Quik staining method. Inhibition of BXD containing serum on cell index（ CI） for proliferation of GC9811-P cells,invasion and metastasis were observed by real time cellular analysis（ RTCA） and Diff-Quik staining method. Results BXD containing serum could obviously inhibit the proliferation of GC9811-P cells. The CI approximated to 0 after 70 h. Most stained Diff-Quik cells died. Cell migration curve showed that 25,50,100 μL / m L BXD containing serums could obviously inhibit the capacities for cell migration of GC9811-P cells in concentration dependent manner. The number of migration cells was reduced more obviously,as compared with 0 μL / m L BXD containing serum（P〈0. 05）. Conclusion BXD containing serums could inhibit the proliferation,invasion and metastasis of GC9811-P cells,which might be associated with blocking peritoneal metastasis of gastric cancer.

关 键 词：人胃癌腹膜高转移潜能细胞株GC9811-P 半夏泻心汤 增殖 侵袭转移 

分 类 号：R285[医药卫生—中药学]