莪术醇对体外多发性骨髓瘤细胞生物学行为的影响  

Effect of Curcumol on the Biological Behavior of Multiple Myeloma Cells

作　　者：孙红[1] 李凌云[2] 周湘明[1] 傅晋翔[3] 

机构地区：[1]苏州大学附属第二医院中医科,江苏215004 [2]苏州大学附属第二医院实验中心,江苏215004 [3]苏州大学附属第二医院血液科,江苏215004

出　　处：《中国中西医结合杂志》2016年第10期1229-1234,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：苏州市科技发展计划资助项目(No.SYSD2812139)

摘　　要：目的观察莪术醇对体外多发性骨髓瘤(multiple myeloma,MM)细胞生物学行为的影响,为莪术醇临床治疗MM提供理论依据。方法以骨髓间充质干细胞(bone marrow derived mesenchymal stem cells,BMSCs)和骨髓瘤8226细胞株(multiple myeloma cell line 8226,RPMI 8226)为研究对象,分为RMPI 8226单独培养组(RMPI 8226)和BMSCs RMPI 8226细胞共培养组(BMSCs+RMPI 8226),分别加入不同浓度(0.1、0.5、1、10μg/m L)的莪术醇,采用流式细胞术检测莪术醇对各组RPMI 8226增殖、周期及凋亡的影响;应用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)检测BMSCs成骨分化基因核因子κb受体活化因子配体(receptor activator of nuclear factorκb ligand,RANKL)及骨保护素(osteoprotegerin,OPG)的表达水平。结果莪术醇可诱导RMPI 8226细胞周期阻滞,且对单独培养组RMPI 8226细胞的周期阻滞明显高于BMSCs共培养组;莪术醇能明显抑制RPMI 8226细胞增殖,诱导RPMI 8226细胞凋亡(均P<0.01),BMSCs可明显降低莪术醇诱导的RPMI 8226细胞凋亡;莪术醇可下调骨髓瘤骨病相关基因RANKL表达,上调OPG表达。结论莪术醇能干扰MM细胞周期,诱导细胞凋亡,在下调RANKL表达的同时,上调OPG表达;BMSCs共培养明显抑制莪术醇诱导的MM细胞凋亡,可能与MM细胞耐药有关。Objective To observe the effect of curcumol on the biological behavior of multiple myeloma（ MM） cells,thus studying its possible mechanisms for MM treatment. Methods Bone marrow mesenchymal stem cells（ BMSCs） and multiple myeloma cell line 8226（ RPMI 8226） were taken as subjects,which were then divided into the RMPI 8226 group（ cultured by RMPI 8226 alone） and the BMSCs ＋ RMPI 8226 group（ cultured by BMSCs and RMPI 8226）. Curcumol in different concentrations（ 0. 1,0. 5,1. 0,10. 0 μg / m L） was added to cells in the two groups respectively. Cell proliferation,cell cycle,and apoptosis induced by curcumol were examined by flow cytometry. The expressions of receptor activator of nuclear factor κb ligand（ RANKL） and osteoprotegerin（ OPG）were detected using reverse transcriptase-polymerase chain reaction（ RT-PCR）. Results Curcumol induced arrested cell cycle of RMPI 8226. The arrest of RMPI 8226 cell cycle was more obviously in the RMPI 8226 group than in the BMSCs ＋ RMPI 8226 group. After curcumol treatment the cell proliferation of RPMI 8226 was significantly inhibited（P〈0. 01） and its apoptosis was increased（P〈0. 01）. Co-cultured with BMSCs decreased curcumol induced apoptosis of RPMI 8226. Curcumol down-regulated the expression of osteogenic differentiation related geneRANKL,and up-regulated the expression of OPG. Conclusions Curcumol disturbed the cell cycle and induced apoptosis of RPMI 8226 cells. Curcumol up-regulated the expression of OPG as well as down-regulated the expression of RANKL. Co-culture with BMSCs could obviously inhibit curcumol induced apoptosis of MM cells,which might be associated with drug resistance of MM cells.

关 键 词：莪术醇 多发性骨髓瘤 生物学行为 耐药 

分 类 号：R285[医药卫生—中药学]