微流控芯片非接触电导法分离检测5种阳离子  

Determination of 5 inorganic cations by microfluidic chip with contactless conductivity detection

作　　者：韩丽萍[1] 陈缵光[1] 

机构地区：[1]中山大学药学院,广州510006

出　　处：《分析试验室》2016年第10期1121-1124,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21375152);广东省科技计划项目(2016B030303002)资助

摘　　要：建立了微流控芯片非接触电导法快速分离检测5种常见阳离子的分析方法。考察了缓冲溶液的种类、浓度和pH、添加剂、检测器的激发电压和激发频率等因素对离子分离及检测的影响。根据离子的峰高和信噪比(S/N),研究了非接触电导检测器的激发电压和激发频率对不同阳离子检测的影响规律。以pH 3.8的15 mmol/L精氨酸(Arg)+15 mmol/L酒石酸+2 mmol/L18-冠醚-6+10%(V/V)甲醇作为运行缓冲液,检测器激发电压100 Vp-p、激发频率40 k Hz,可在70 s内实现NH^+_4,K^+,Na^+,Mg^(2+)和Ca^(2+)的分离检测,5种离子的迁移时间重现性RSD均在1.5%以内。结果还表明,非接触电导检测器激发电压和激发频率对5种阳离子检测的影响规律和最佳检测条件基本相同。A method for the rapid determination of 5 inorganic cations by microfluidic chip with contactless conductivity detection was developed. In this study,a number of main factors related to separation and detection of cations were investigated. And the separation conditions were optimized firstly in microchip, such as background electrolyte species and concentration,pH and organic additives. Moreover,the peak heights and signal-noise ratios（ S/N） of different cations were recorded with contactless conductivity detector. Thus,the effects of excitation voltage and excitation frequency on detection of cations were studied. Good results in microchip devices were achieved as follows： 15 mmol/L arginine（ Arg）,15 mmol/L tartaric acid,2 mmol/L18-crown-6 and 10%（ V/V） methyl alcohol at pH 3. 8. The excitation voltage of 100 Vp-pand excitation frequency of 40 k Hz for detection were used in all experiments. Under the optimal conditions,the mixture of NH^＋_4,K^＋,Na^＋,Mg^（2＋）and Ca^（2＋）were separated and detected in 70 s. The RSDs of migration time were less than 1. 5%. In addition,it was found that the best excitation voltage and excitation frequency for the detection of5 cations were basically the same.

关 键 词：微流控芯片 非接触电导检测 无机阳离子 

分 类 号：O657.1[理学—分析化学]