早期HIV-1感染者KIR3 DL1阳性CD8细胞表型和功能的探索研究  

Phenotypes and HIV-1-specific T cell responses of KIR3DL1 positive CD8 cells in patients with early HIV-1 infection

作　　者：张欣[1] 粟斌[1] 陆小凡[1] 刘志英[1] 计云霞[1] 王蕊[1] 张彤[1] 吴昊[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心、艾滋病研究北京市重点实验室,北京100069

出　　处：《中华微生物学和免疫学杂志》2016年第9期699-703,共5页Chinese Journal of Microbiology and Immunology

基　　金：艾滋病研究北京市重点实验室（BZ0089）;国家自然科学基金（8157080022）;北京市卫生系统高层次卫生技术人才培养计划（2015-3-102）

摘　　要：目的：探讨 KIR3DL1受体在早期 HIV-1感染者 CD8细胞亚群的表达特点和KIR3DL1＋CD8＋细胞HIV-1特异性应答能力。方法用流式细胞术检测56例HIV-1阴性对照者和32例早期HIV-1感染者的CD8细胞亚群；同时用HIV-1 Gag多肽库刺激HIV-1感染者的外周血单个核细胞（ PBMCs）,用胞内细胞因子染色技术检测CD8细胞分泌IFN-γ水平。结果 HIV-1阴性对照者和HIV-1感染者CD8细胞中KIR3DL1＋CD8＋细胞比例的中位数分别为1.45%（0.12%-8.4%）和0.82%（0.14%-6.14%）,差异无统计学意义。 KIR3DL1受体在HIV-1阴性者和HIV-1感染者CD8终端效应细胞的表达比例平均值分别是（4.55±3.84）%和（6.71±8.50）%,显著高于在CD8效应记忆细胞的表达比例,其平均值分别是（0.50±0.59）%和（1.18±1.39）%（P〈0.001）。而且,早期HIV-1感染者CD8效应记忆细胞中KIR3DL1阳性细胞比例显著高于HIV-1阴性者（P=0.0012）。早期HIV-1感染者的KIR3DL1＋CD8＋终端效应细胞比例与HIV-1载量呈正比（rs=0.576,P=0.0009）；而且,HIV-1载量≤4.0log 的 HIV-1感染者 KIR3DL1＋CD8＋终端效应细胞的比例显著小于病毒载量〉4.0log的感染者（P=0.002）。 HIV-1感染者KIR3DL1＋CD8＋细胞诱导产生HIV-1-特异性IFN-γ的水平远低于KIR3DL1-CD8＋细胞。结论 KIR3DL1受体主要表达在CD8终端效应细胞,HIV-1病毒血症高时KIR3DL1受体在终端效应细胞的表达比例高；表达 KIR3DL1受体的 CD8细胞诱导产生的HIV-1特异性T细胞的应答能力低。Objective To investigate the phenotypes and the HIV-1-specific T cell responses of KIR3DL1 positive CD8 cells in patients with early HIV-1 infection. Methods Fifty-six HIV-1 antibody negative individuals and thirty-two patients with early HIV-1 infection were enrolled in the study. Fluores-cence-activated cell sorting （FACS） was performed to detect the phenotypes of KIR3DL1 receptor expressed on the surface of CD8 cells. The levels of IFN-γwere measured by intracellular cytokine staining assay after the PBMCs were stimulated with an HIV-1 Gag peptide pool. Results The percentages of KIR3DL1＋CD8 T cells in HIV-1 negative individuals and patients with early HIV-1 infection were 1. 45% （0. 12%-8. 4%） and 0. 82% （0. 14%-6. 14%）, respectively, and there was no significant difference between them. The percentages of KIR3DL1＋CD8 Temra cells in HIV-1 negative individuals and patients with early HIV-1 infec-tion were （4. 55±3. 84）% and （6. 71±8. 50）%, respectively, which were significantly higher than the per-centages of KIR3DL1＋CD8 Tem cells, which were （0. 50±0. 59）% and （1. 18±1. 39）%, respectively （all P〈0. 01）. Moreover, the percentages of KIR3DL1＋CD8 Tem cells in patients with early HIV-1 infectionwere higher than those in HIV-1 negative individuals （P=0. 001 2）. The percentage of KIR3DL1＋CD8 Temra cells was positively correlated with the HIV-1 viral load in patients with early HIV-1 infection （ rs=0. 576,P=0. 000 9）. The percentages of KIR3DL1＋CD8 Temra cells in HIV-1 patients, whose viral loads were larger than 4. 0log, were much higher than those in HIV-1 patients with viral loads less than 4. 0 log （P=0. 002）. Additionally, the levels of IFN-γsecreted by KIR3DL1 positive CD8 cells were much lesser than those secreted by KIR3DL1 negative CD8 cells （P〈0. 000 1）. Conclusion The receptor of KIR3DL1 was mainly expressed on CD8 Temra cells in both HIV-1 negative subjects and patients with early HIV-1 infec-tion. High HIV-1 viremia was associated wit

关 键 词：早期HIV-1感染 KIR3DL1受体 CD8细胞 

分 类 号：R512.91[医药卫生—内科学]