马桑水提取物对烧伤创面感染常见3种耐药菌的抑制作用  

Inhibition of Coriaria Sinica Maxim's extract on burn wound infections with common three kinds of resistant bacteria

作　　者：黄德斌[1] 胡泽华[1] 余昭芬[1] 陈薛妃 黄谨[1] 

机构地区：[1]湖北民族学院医学院,湖北恩施445000

出　　处：《中国药理学通报》2016年第10期1388-1394,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81360654)

摘　　要：目的探讨马桑水提取物(CSME)对烧伤创面感染耐药金黄色葡萄球菌(MRSA)、铜绿假单胞菌(RPA)和大肠埃希菌(RECO)的抑制作用。方法将耐药菌株经MH琼脂平板培养,提取耐药菌株基因并鉴定质控菌的耐药基因(如mec A、mex B、mer A、qac E明显△1-sull、tnp U/A及mex B等)后,耐药菌菌样接种并测定CSME的相应抑菌谱、最小抑菌浓度(MIC)、不同浓度的敏感率和抑菌曲线,最后与抗生素抑菌作用比较,明确其敏感率。结果 CSME对MESA、RPA和RECO的MIC分别为62.5、125、250 g·L^(-1);CSME对这3种耐药菌抑制率呈现浓度依赖性,且CSME各个不同浓度对RECO抑制率明显低于MRSA和RPA(P<0.05);在MIC时,MRSA对于羧苄西林、头孢唑林、红霉素等耐药率明显高于CSME(P<0.05);CSME对MRSA的抑菌圈明显小于头孢曲松、头孢吡肟、亚胺培南,而大于其他抗生素(P<0.05);对RPA的抑菌圈明显小于羧苄西林,而大于其他抗生素(P<0.05);对RECO的抑菌圈明显小于头孢曲松、头孢吡肟、亚胺培南、环丙沙星、呋喃西林,而大于其他抗生素(P<0.05)。结论 CSME对烧伤创面感染的常见这3种耐药菌具有明显抑制作用,提示CSME有可能成为有效控制烧伤创面多重耐药菌混合感染的可选药物之一。Aim To explore the inhibition of Sinica Maxim′s extract（ CSME） on resistant infections of burn wounds,such as the methicillin-resistant staphylococcus aureus （ MRSA ） , resistant pseudomonas aeruginosa （RPA） and resistant escherichia coli（RECO）. Meth-ods The resistant strains were cultured by MH agar plates. After resistance genes of quality control strains were extracted and appraised, such as mecA, mexB, merA, qacE△1-sull, tnpU/A and mexB, etc, and then,some projects of CSME were detected,such as the antibacterial spectrum, the minimum inhibitory con-centration（MIC）, different concentrations of sensitive rate and inhibition curves, etc. Finally, these results were compared with the inhibitory effects of some anti-biotics to determine the sensitivity rates of CSME. Re-sults The MIC of CSME was 62. 5 ,125 ,250 g ·L^-1 respectively on the MESA, RPA and RECO. The inhi-bition rates of CSME appeared concentration-dependent on these three kinds of resistant bacteria,and the inhi-bition rates of the multi-concentration CSME on RECO were significantly lower than on MRSA and RPA （ P〈0. 05）. While in MIC,the resistance rates of MRSA on carbenicillin, cefazolin, erythromycin were significant-ly higher than those of CSME（P〈0. 05）; The inhibi-tion zones of CSME were significantly smaller than those of ceftriaxone, cefepime, imipenem, but greater than those of other antibiotics（ P〈0. 05 ）; The inhibi-tion zones of CSME on RPA were significantly smaller than those of carbenicillin, and greater than those of other antibiotics （ P 〈0. 05 ） . The inhibition zones of CSME on RECO were significantly smaller than those of ceftriaxone,cefepime,imipenem,ciprofloxacin,nitro-furazone,and greater than those of other antibiotics （ P〈0. 05 ） . Conclusions CSME has a significant inhi-bition on burn wound infection with these three kinds of resistant bacteria,such as MRSA,RPA and RECO. It is prompted that CSME could become one of the effective drugs to control burn wound infections with

关 键 词：马桑水提取物 烧伤感染 耐药菌 抑制作用 敏感率 抑菌曲线 

分 类 号：R284.1[医药卫生—中药学] R378.11[医药卫生—中医学]